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Application of Genetically Encoded Fluorescent Nitric Oxide (NO&#8226;) Probes, the geNOps, for Real-time Imaging of NO&#8226; Signals in Single Cells
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An isolated single-particle-based SECM tip interface for single-cell NO sensing.

Jiening Wu1, Yafang Gao1, Na Pan1

  • 1Key Laboratory of Beijing on Regional Air Pollution Control, Department of Environmental Science, Beijing University of Technology, Beijing, 100124, PR China.

Biosensors & Bioelectronics
|January 1, 2023
PubMed
Summary
This summary is machine-generated.

Researchers developed a new Scanning Electrochemical Microscopy (SECM) tip for real-time nitric oxide (NO) monitoring in single cells. This advanced probe offers high sensitivity and selectivity for detecting cellular NO fluctuations.

Keywords:
A single nanoparticleNitric oxideSECMSensingSingle cell

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Area of Science:

  • Electrochemistry
  • Cell Biology
  • Nanotechnology

Background:

  • Nitric oxide (NO) is crucial for cellular metabolism but difficult to monitor in real-time due to its transient nature.
  • Scanning Electrochemical Microscopy (SECM) is valuable for single-cell analysis, offering electrochemical current measurements via tip microelectrode scanning.
  • Enhancing SECM tip interface properties can significantly improve electrochemical response and analytical capabilities.

Purpose of the Study:

  • To develop an advanced SECM tip for sensitive and selective in situ real-time monitoring of nitric oxide (NO).
  • To investigate the NO release dynamics from single MCF-7 cells under cadmium stimulation using the novel SECM probe.
  • To demonstrate the utility of single-particle-based tips in SECM for cellular analysis.

Main Methods:

  • Fabrication of an SECM tip modified with a platinum single nanoparticle (Pt NP) and fluorinated self-assembled monolayers (SAMs).
  • Utilizing the modified tip for Scanning Electrochemical Microscopy (SECM) to monitor NO concentration and perform morphological imaging.
  • Applying the SECM probe to detect NO fluctuations in single MCF-7 cells stimulated with cadmium (Cd).

Main Results:

  • The modified SECM tip demonstrated high sensitivity (164.7 μA/μM·cm²) and selectivity for NO detection.
  • The Pt NP and fluorinated SAMs interface provided efficient catalysis, high mass transport, and hydrophobic antifouling properties.
  • The probe successfully detected dynamic changes in NO release from single MCF-7 cells upon cadmium exposure.

Conclusions:

  • The developed Pt NP-based SECM tip with a fluorinated SAM interface offers a highly sensitive and selective method for NO monitoring.
  • This approach enables real-time tracking of NO fluctuations in single cells, providing insights into cellular responses.
  • Single-particle-based tips represent a promising advancement for SECM applications in cellular electrochemistry and biological studies.