Two-dimensional speckle tracking-derived global longitudinal strain in healthy Doberman Pinschers: method evaluation,...

Area of Science:

• Veterinary Cardiology
• Echocardiography
• Cardiac Mechanics

Background:

• Assessing systolic function in Doberman Pinschers (DP) using two-dimensional speckle tracking (2D-ST) derived global longitudinal strain (GLS) requires establishing reference values in healthy individuals.
• Feasibility and observer variability of 2D-ST-derived GLS need evaluation using different software programs.

Purpose of the Study:

• To determine the feasibility and observer-related measurement variability of 2D-ST-derived GLS in healthy Doberman Pinschers.
• To establish reference values for GLS derived from apical four-chamber (GLS_A4C), two-, and three-chamber views, as well as an average of all three views (GLS_AV).
• To compare GLS measurements between AutoSTRAIN and 2D Cardiac Performance Analysis software.

Main Methods:

• Prospective study involving 120 healthy Doberman Pinschers.
• Determination and comparison of GLS_A4C, GLS_AV, and other views using AutoSTRAIN and 2D Cardiac Performance Analysis.
• Assessment of intra- and inter-observer measurement variability using coefficient of variation.

Main Results:

• Both software programs yielded similar and positively correlated GLS values, with a small difference in GLS_AV between them.
• AutoSTRAIN showed no significant difference between GLS_AV and GLS_A4C, while 2D Cardiac Performance Analysis did.
• Software-specific reference values were established; AutoSTRAIN demonstrated lower measurement variability compared to 2D Cardiac Performance Analysis.

Conclusions:

• Two-dimensional speckle tracking derived GLS is a feasible method for evaluating systolic function in Doberman Pinschers with low measurement variability.
• GLS_AV is recommended when possible, but GLS_A4C can serve as a viable alternative.
• Reference values and variability metrics support the clinical application of 2D-ST for GLS assessment in this breed.