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Updated: Aug 15, 2025

A Bioinformatics Pipeline to Accurately and Efficiently Analyze the MicroRNA Transcriptomes in Plants

A Bioinformatics Pipeline to Accurately and Efficiently Analyze the MicroRNA Transcriptomes in Plants

Published on: January 21, 2020

Upcoming progress of transcriptomics studies on plants: An overview.

Parul Tyagi¹, Deeksha Singh¹, Shivangi Mathur¹

¹Plant Molecular Biology Lab, Department of Botany, Faculty of Science, Dayalbagh Educational Institute, Agra, India.

Frontiers in Plant Science

|January 2, 2023

Summary

RNA-Sequencing (RNA-Seq) is a powerful tool for studying plant transcriptomes, aiding in gene discovery and understanding biological processes. This review explores RNA-Seq technologies and their applications for functional gene mining in plants.

Keywords:

NGS gene function molecular markers secondary metabolites transcriptome analysis

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Isolation and Transcriptome Analysis of Plant Cell Types

Isolation and Transcriptome Analysis of Plant Cell Types

Published on: April 7, 2023

Area of Science:

Plant Biology
Genomics
Molecular Biology

Background:

Transcriptome sequencing, or RNA-Sequencing (RNA-Seq), is a high-resolution, sensitive, and high-throughput next-generation sequencing (NGS) approach.
RNA-Seq enables the assembly of RNA transcripts from various samples and developmental stages, crucial for studying non-model organisms.
It is significant for gene prediction, functional analysis, and understanding gene ontology mechanisms.

Purpose of the Study:

To review existing and forthcoming strategies for improving transcriptome sequencing technologies.
To focus on functional gene mining in various plants using RNA-Seq technology.
To examine RNA-Seq technologies in terms of their principles, development, and scientific applications.

Main Methods:

Review of transcriptome sequencing and analysis techniques.
Examination of advancements in RNA-Seq technology for transcriptome characterization.
Focus on strategies for improving RNA-Seq for functional gene mining.

Main Results:

RNA-Seq provides insights into gene predictions and functional analysis, enhancing understanding of biological processes.
Transcriptomics research aids in understanding functional genes and regulatory processes in plants.
Advancements allow for efficient characterization of transcriptomes in distinct cell types.

Conclusions:

RNA-Seq is a vital technique for plant biology research, improving gene ontology understanding.
It supports enhanced breeding selection and cultivation practices through better understanding of functional genes.
The review highlights the importance and future directions of RNA-Seq in plant functional genomics.