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Chorionic villus sampling: improved method for preparation of karyotypes after short-term incubation
G L Terzoli1, F Lalatta, F Gilbert
1Laboratorio di Citogenetica Instituti Clinici di Perfezionamento, Milano, Italy.
Prenatal Diagnosis
|July 1, 1987
Abstract:
A method for the isolation and trypsin-Giemsa banding of metaphases obtained after short-term incubation (48 h) of cytotrophoblast cells from chorionic villus sample is described. A new slide-making instrument, developed expressly to enhance the spreading of chromosomes from metaphases released from small tissue pieces, is responsible for the increase yield of analysable metaphases in this protocol.