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Related Experiment Video

Updated: Aug 15, 2025

Injection of Hydrogel Biomaterial Scaffolds to The Brain After Stroke
09:41

Injection of Hydrogel Biomaterial Scaffolds to The Brain After Stroke

Published on: October 1, 2020

5.3K

Particle hydrogels decrease cerebral atrophy and attenuate astrocyte and microglia/macrophage reactivity after

Elias Sideris1, Sophia Kioulaphides2, Katrina Wilson2

  • 1Department of Chemical and Biomolecular Engineering, University of California Los Angeles, Los Angeles, CA, United States.

Advanced Therapeutics
|January 2, 2023
PubMed
Summary
This summary is machine-generated.

Injecting a novel hydrogel into stroke-affected brain areas in mice reduced brain damage and promoted repair. This innovative stroke therapy modulated brain cells, decreasing atrophy and preserving neural connections for better recovery.

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Area of Science:

  • Neuroscience
  • Biomaterials Science
  • Regenerative Medicine

Background:

  • Stroke-related disabilities affect many individuals, with reactive astrocytes and pro-inflammatory microglia contributing to secondary brain damage and hindering repair.
  • These same glial cells, however, also possess pro-repair capabilities crucial for endogenous recovery and brain plasticity.

Purpose of the Study:

  • To investigate the therapeutic potential of a hyaluronic acid-based microporous annealed particle (MAP) hydrogel for stroke recovery.
  • To assess the impact of MAP hydrogel injection into the stroke core on glial cell phenotypes and brain tissue preservation.

Main Methods:

  • Direct injection of MAP hydrogel into the stroke core in a mouse model.
  • Analysis of astrocyte reactivity and microglial activation states post-injection.
  • Quantification of cerebral atrophy and preservation of neurofilament-200 (NF200) axonal bundles.

Main Results:

  • MAP hydrogel injection significantly reduced the percentage of highly reactive astrocytes.
  • An increased percentage of alternatively activated (pro-repair) microglia was observed.
  • Reduced cerebral atrophy and preservation of NF200 axonal bundles were evident post-treatment.
  • MAP hydrogel promoted reparative astrocyte infiltration and subsequent axonal penetration into the lesion site.

Conclusions:

  • Direct injection of porous MAP hydrogel into the stroke core can mitigate secondary brain damage.
  • This approach effectively modulates astrocytes and microglia towards a pro-repair phenotype, promoting endogenous repair mechanisms.
  • The findings suggest a clinically relevant therapeutic strategy for reducing cerebral atrophy and enhancing brain plasticity after stroke.