Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Designer indicators for two-photon recording of subthreshold voltage dynamics.

Nature methods·2026
Same author

Inactivation of the Phosphatase Activity of Soluble Epoxide Hydrolase Modulates SIRT3 and Attenuates Experimental Pulmonary Hypertension.

Comprehensive Physiology·2026
Same author

Chronically implantable μLED arrays for optogenetic cortical surface stimulation in mice.

Nature communications·2026
Same author

Dual AAV gene therapy achieves recovery of hearing and auditory processing in a DFNB16 mouse model.

Clinical and translational medicine·2026
Same author

The Geometry of Layer 2/3 Cortical Sound Processing in Slow Wave Sleep.

Advanced science (Weinheim, Baden-Wurttemberg, Germany)·2025
Same author

Sound feature representations decorrelate across the mouse auditory pathway.

PLoS biology·2025

Related Experiment Video

Updated: Aug 15, 2025

Functional Neuroimaging Using Ultrasonic Blood-brain Barrier Disruption and Manganese-enhanced MRI
08:36

Functional Neuroimaging Using Ultrasonic Blood-brain Barrier Disruption and Manganese-enhanced MRI

Published on: July 12, 2012

15.1K

Enhanced perceptual task performance without deprivation in mice using medial forebrain bundle stimulation.

Antonin Verdier1, Noémi Dominique2, Déborah Groussard2

  • 1Institut de l'Audition, Institut Pasteur, Université de Paris, INSERM, 75012 Paris, France.

Cell Reports Methods
|January 2, 2023
PubMed
Summary

Electrical stimulation of the medial forebrain bundle (MFB) offers a novel method for training animals in neuroscience tasks. This approach enhances motivation and performance without the need for water deprivation, improving animal welfare.

Keywords:
Go/No-goauditionbehaviorlever pressinglickingmedial forebrain bundlemotivationmouseperceptionperceptual decision-makingreinforcement learningsatiation

More Related Videos

Optogenetic Manipulation of Neuronal Activity to Modulate Behavior in Freely Moving Mice
14:40

Optogenetic Manipulation of Neuronal Activity to Modulate Behavior in Freely Moving Mice

Published on: October 27, 2020

18.4K
Long-term Potentiation of Perforant Pathway-dentate Gyrus Synapse in Freely Behaving Mice
11:13

Long-term Potentiation of Perforant Pathway-dentate Gyrus Synapse in Freely Behaving Mice

Published on: November 29, 2013

14.2K

Related Experiment Videos

Last Updated: Aug 15, 2025

Functional Neuroimaging Using Ultrasonic Blood-brain Barrier Disruption and Manganese-enhanced MRI
08:36

Functional Neuroimaging Using Ultrasonic Blood-brain Barrier Disruption and Manganese-enhanced MRI

Published on: July 12, 2012

15.1K
Optogenetic Manipulation of Neuronal Activity to Modulate Behavior in Freely Moving Mice
14:40

Optogenetic Manipulation of Neuronal Activity to Modulate Behavior in Freely Moving Mice

Published on: October 27, 2020

18.4K
Long-term Potentiation of Perforant Pathway-dentate Gyrus Synapse in Freely Behaving Mice
11:13

Long-term Potentiation of Perforant Pathway-dentate Gyrus Synapse in Freely Behaving Mice

Published on: November 29, 2013

14.2K

Area of Science:

  • Neuroscience
  • Animal Behavior

Background:

  • Perceptual decision-making tasks are crucial in neuroscience research.
  • Current methods often rely on water deprivation, posing challenges for animal welfare and limiting experimental duration due to satiation.

Purpose of the Study:

  • To introduce and evaluate medial forebrain bundle (MFB) electrical stimulation as an alternative reward system for perceptual decision-making tasks.
  • To assess if MFB stimulation can maintain stable motivation for extended experimental sessions without compromising animal well-being.

Main Methods:

  • Auditory discrimination tasks were employed using either licking or lever press as a behavioral report.
  • Two groups of mice were compared: one receiving MFB electrical stimulation and the other subjected to water deprivation.
  • Performance metrics including learning speed, accuracy, trial number, psychometric parameters, and response times were analyzed.
  • Metabolic and behavioral stress indicators were monitored in both groups.

Main Results:

  • Animals motivated by MFB stimulation learned auditory discrimination tasks as quickly as water-deprived mice.
  • MFB-stimulated animals achieved higher accuracy, particularly in more challenging tasks, completing up to 4,500 trials per session without motivational decline.
  • MFB stimulation did not alter sensory behavior, preserving psychometric parameters and response times.
  • MFB-stimulated mice showed no signs of metabolic or behavioral stress compared to water-deprived controls.

Conclusions:

  • Medial forebrain bundle (MFB) electrical stimulation is a viable and effective alternative to water deprivation for motivating animals in perceptual decision-making tasks.
  • This method supports enhanced task performance and extended experimental sessions while safeguarding animal welfare.
  • MFB stimulation represents a promising advancement for neuroscience research, enabling more robust and ethical behavioral studies.