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Pyrene-Based Self-Assembling Peptide for Ratiometric Detection of Heparin
Liang Shao1, Hongwen Yu1,2, Jiaqi Song1
1Department of Biophysics, School of Basic Medical Sciences, Health Science Centre, Xi'an Jiaotong University, Shaanxi, 710061, P. R. China.
Chembiochem : a European Journal of Chemical Biology
|January 2, 2023
Summary
A novel fluorescent peptide, PyFFRRR, enables simple and selective detection of heparin levels. This method is crucial for preventing adverse reactions from excessive heparin anticoagulant use.
Area of Science:
- Biochemistry
- Analytical Chemistry
- Materials Science
Background:
- Heparin is a vital anticoagulant, but its excessive levels can lead to severe adverse events.
- Accurate and convenient heparin detection is essential for safe clinical practice.
Purpose of the Study:
- To develop a simple, selective, and efficient method for detecting heparin levels.
- To design a pyrene-based self-assembling fluorescent peptide probe for heparin detection.
Main Methods:
- Design and synthesis of a pyrene-based self-assembling fluorescent peptide (PyFFRRR).
- Investigation of the binding mechanism between PyFFRRR and heparin using spectral and simulation tools.
- Application of PyFFRRR for ratiometric detection of heparin in human serum samples.
Main Results:
- PyFFRRR demonstrated strong binding with heparin via electrostatic interactions between guanidine and sulfate groups.
- Self-assembly of PyFFRRR upon heparin binding induced a spectral shift from blue monomer to green excimer fluorescence.
- The probe exhibited excellent sensitivity and selectivity for ratiometric heparin detection.
Conclusions:
- PyFFRRR serves as an effective fluorescent probe for the ratiometric detection of heparin.
- This method offers a promising approach for monitoring heparin levels in clinical settings.
- The developed probe facilitates simple, selective, and efficient heparin detection, enhancing patient safety.
