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Bacterial nanocellulose production using Cantaloupe juice, statistical optimization and characterization
Noura El-Ahmady El-Naggar1, A B Abeer Mohammed2, Sahar E El-Malkey2
1Department of Bioprocess Development, Genetic Engineering and Biotechnology Research Institute, City of Scientific Research and Technological Applications (SRTA-City), New Borg El-Arab City, Alexandria, 21934, Egypt. nouraalahmady@yahoo.com.
Scientific Reports
|January 2, 2023
Summary
Bacillus sp. strain SEE-3 efficiently produces bacterial nanocellulose (BNC) for biomedical uses. Optimization studies identified key conditions for maximizing BNC yield, achieving 20.31 g/L.
Area of Science:
- Biotechnology
- Materials Science
- Biochemistry
Background:
- Bacterial nanocellulose (BNC) is a versatile biomaterial with extensive biomedical applications.
- Screening diverse bacterial strains is crucial for identifying high-yield BNC producers.
Purpose of the Study:
- To screen bacterial strains for optimal BNC production.
- To characterize the physicochemical properties of the produced BNC.
- To optimize BNC production using statistical design methods.
Main Methods:
- Screening of ten bacterial strains, with Bacillus sp. strain SEE-3 identified as a potent producer.
- Characterization of BNC using SEM, TEM, XRD, FTIR, and TGA.
- Optimization of BNC production using Plackett-Burman and Central Composite Designs.
Main Results:
- Bacillus sp. strain SEE-3 produced BNC with a crystallinity of 79.58% and high thermal stability.
- SEM and TEM revealed fiber-shaped nanoparticles (20.12–47.36 nm) and needle-shaped particles (30–40 nm x 560–1400 nm).
- Optimized conditions yielded a maximum BNC production of 20.31 g/L.
Conclusions:
- Bacillus sp. strain SEE-3 is a promising candidate for industrial BNC production.
- The characterized BNC possesses suitable properties for biomedical applications.
- Statistical optimization significantly enhanced BNC yield.
