Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Genetic Screens

Genetic Screens

Genetic screens are tools used to identify genes and mutations responsible for phenotypes of interest. Genetic screens help identify individuals or a group of people at risk of developing genetic diseases and help them with early intervention, targeted therapy, and reproductive options.
Forward genetic screens
Forward or “classical” genetic screens involve creating random mutations in an organism’s DNA using radiation, mutagens, or insertion of additional bases, which result in visible changes...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Virome-wide ubiquitin ligase discovery reveals diverse mechanisms of immune evasion.

Science (New York, N.Y.)·2026

Same author

A viral ORFeome library for systems-level genetic dissection of host-pathogen interactions.

Cell·2026

Same author

Persistence of memory: lifespan dynamics of the human antiviral antibody reactome.

Nature communications·2026

Same author

Systems-Scale Structural Modeling Reveals the Germline Architecture of Immunodominance.

bioRxiv : the preprint server for biology·2026

Same author

Integrative Molecular Analyses of Inflammatory and Autoimmune Signals in Cardiac Sarcoidosis.

Circulation·2026

Same author

A multiplexed, systems-based approach for prediction of antibody neutralization breadth for soluble human receptors.

Journal of immunology (Baltimore, Md. : 1950)·2026

Search research articles

Related Experiment Video

Updated: Jun 15, 2026

A High Throughput MHC II Binding Assay for Quantitative Analysis of Peptide Epitopes

A High Throughput MHC II Binding Assay for Quantitative Analysis of Peptide Epitopes

Published on: March 25, 2014

High-throughput, targeted MHC class I immunopeptidomics using a functional genetics screening platform.

Peter M Bruno^1,2, Richard T Timms^1,2,3, Nouran S Abdelfattah^1,2

¹Department of Genetics, Harvard Medical School and Division of Genetics, Brigham and Women's Hospital, Boston, MA, USA.

Nature Biotechnology

|January 2, 2023

Summary

This summary is machine-generated.

EpiScan is a new method for identifying CD8+ T cell epitopes, accelerating the development of personalized immunotherapeutics. This technique efficiently screens large peptide libraries, improving the discovery of crucial immune targets.

More Related Videos

Immunopeptidomics: Isolation of Mouse and Human MHC Class I- and II-Associated Peptides for Mass Spectrometry Analysis

Immunopeptidomics: Isolation of Mouse and Human MHC Class I- and II-Associated Peptides for Mass Spectrometry Analysis

Published on: October 15, 2021

Author Spotlight: Finding New Therapeutic Targets for Malignant Peripheral Nerve Sheath Tumor Through Genome-Scale shRNA Screens

Author Spotlight: Finding New Therapeutic Targets for Malignant Peripheral Nerve Sheath Tumor Through Genome-Scale shRNA Screens

Published on: August 25, 2023

Related Experiment Videos

Last Updated: Jun 15, 2026

A High Throughput MHC II Binding Assay for Quantitative Analysis of Peptide Epitopes

A High Throughput MHC II Binding Assay for Quantitative Analysis of Peptide Epitopes

Published on: March 25, 2014

Immunopeptidomics: Isolation of Mouse and Human MHC Class I- and II-Associated Peptides for Mass Spectrometry Analysis

Immunopeptidomics: Isolation of Mouse and Human MHC Class I- and II-Associated Peptides for Mass Spectrometry Analysis

Published on: October 15, 2021

Author Spotlight: Finding New Therapeutic Targets for Malignant Peripheral Nerve Sheath Tumor Through Genome-Scale shRNA Screens

Author Spotlight: Finding New Therapeutic Targets for Malignant Peripheral Nerve Sheath Tumor Through Genome-Scale shRNA Screens

Published on: August 25, 2023

Area of Science:

Immunology
Computational Biology
Biotechnology

Background:

Identifying CD8+ T cell epitopes is crucial for developing immunotherapeutics.
Current methods for Major Histocompatibility Complex class I (MHC class I) ligand discovery are slow, require specialized expertise, and lack scalability for specific protein interrogation.

Purpose of the Study:

To present EpiScan, a novel method for large-scale MHC class I ligand discovery.
To improve the efficiency and scope of CD8+ T cell epitope identification for immunotherapeutic development.

Main Methods:

EpiScan utilizes surface MHC class I levels as a readout for peptide-MHC class I binding.
Oligonucleotide synthesis enables the design of large peptide pools (>100,000 peptides) for high-throughput screening.
EpiScan was used to analyze peptide-MHC binding, including the role of cysteine, affinity hierarchies, and viral proteomes.

Main Results:

EpiScan facilitates large-scale MHC class I screening by leveraging genetically encoded peptides.
The study revealed that cysteine increases the number of predicted MHC class I ligands by 9-21%.
EpiScan Predictor, developed from these data, demonstrates performance comparable to existing algorithms while accurately accounting for cysteine-containing peptides.

Conclusions:

EpiScan significantly accelerates CD8+ T cell epitope discovery.
The method enhances the prediction of MHC class I ligands, particularly those containing cysteine.
This advancement is vital for the development of precise, individual-specific immunotherapeutics.