Related Experiment Video
Updated: Aug 15, 2025
Determination of Regulatory T Cell Subsets in Murine Thymus, Pancreatic Draining Lymph Node and Spleen Using Flow Cytometry
Published on: February 27, 2019
Protocol to quantify and characterize contact between T cells and antigen-presenting cells in the antigen-draining
Naoya Tatsumi1, Alejandro Davila-Pagan1, Yosuke Kumamoto1
1Center for Immunity and Inflammation, Rutgers New Jersey Medical School, Newark, NJ 07103, USA; Department of Pathology, Immunology and Laboratory Medicine, Rutgers New Jersey Medical School, Newark, NJ 07103, USA.
Physical contact between T cells and antigen-presenting cells (APCs) is essential for priming antigen-specific T cells, but quantitating the antigen-dependent T cell-APC contact can be laborious. Here, we present a simple flow-cytometry-based protocol for quantitating T cell-APC contacts in the antigen-draining lymph node in mice immunized with ovalbumin (OVA). This protocol quantifies the contact between adoptively transferred OVA-specific TCR transgenic CD4T (OT-II) cells and dendritic cell (DC) subsets. This approach can be applied to other types of intercellular interactions between T cells and APCs. For complete details on the use and execution of this protocol, please refer to Tatsumi et al. (2021).1.
Physical contact between T cells and antigen-presenting cells (APCs) is essential for priming antigen-specific T cells, but quantitating the antigen-dependent T cell-APC contact can be laborious. Here, we present a simple flow-cytometry-based protocol for quantitating T cell-APC contacts in the antigen-draining lymph node in mice immunized with ovalbumin (OVA). This protocol quantifies the contact between adoptively transferred OVA-specific TCR transgenic CD4T (OT-II) cells and dendritic cell (DC) subsets. This approach can be applied to other types of intercellular interactions between T cells and APCs. For complete details on the use and execution of this protocol, please refer to Tatsumi et al. (2021).1.
