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Updated: Aug 15, 2025
A Method of Targeted Cell Isolation via Glass Surface Functionalization
Published on: September 20, 2016
Protocol for cell surface biotinylation of magnetic labeled and captured human peripheral blood mononuclear cells
Serife Ayaz-Guner1, Mustafa Burak Acar2, Dudu Boyvat3
1Department of Molecular Biology and Genetics, Izmir Institute of Technology, Izmir 35430, Türkiye; Department of Molecular Biology and Genetics, Faculty of Life and Natural Science, Abdullah Gül University, Kayseri 38080, Türkiye.
Abstract:
Analysis of the surfaceome of a blood cell subset requires cell sorting, followed by surface protein enrichment. Here, we present a protocol combining magnetically activated cell sorting (MACS) and surface biotinylation of the target cell subset from human peripheral blood mononuclear cells (PBMCs). We describe the steps for isolating target cells and their in-column surface biotinylation, followed by isolation and mass spectrometry analysis of biotinylated proteins. The protocol enables in-column surface biotinylation of specific cell subsets with minimal membrane disruption.
