Texturization of pea protein isolate by micro compounding

Twin-screw micro compounding is introduced as a novel technique to process and characterize small plant protein samples under conditions that are relevant for meat analogue processing. Small samples of pea protein isolate (PPI) (5 cm³, corresponding to ∼7 g of hydrated sample) are batch-processed at water contents between 40 and 70 % w/w and temperatures between 90 and 120 °C. Screw speed (100-400 rpm) and residence time (1-9 min) are varied resulting in values of the specific mechanical energy (SME) between ∼20 and 2000 kJ/kg, which is the range relevant for plant protein extrusion. Micro compounding process data provides information on several aspects of the rheological behavior of PPI. Shear thinning behavior is observed for PPI. The viscosity of the PPI during micro compounding was found to exponentially decrease with water content. The temperature dependence is consistent with an Arrhenius-type model. The extruded strands (length: ∼15 cm; diameter: 3.0 ± 0.2 mm) are characterized by scanning electron microscopy (SEM), differential solubility, water holding capacity (WHC), and texture profile analysis (TPA). The hardness as determined from TPA increases linearly with screw speed and residence time, jumps to higher values above the denaturation temperature of the PPI and decreases exponentially with the water content during processing. Micro compounding is found to be a useful technique to convert small plant protein samples at water contents between about 40 and 60 % w/w into texturized matrices and investigate the rheological behavior of plant protein isolates under conditions that are relevant for extrusion processing.