Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

CRISPR01:59

CRISPR

52.6K
Genome editing technologies allow scientists to modify an organism’s DNA via the addition, removal, or rearrangement of genetic material at specific genomic locations. These types of techniques could potentially be used to cure genetic disorders such as hemophilia and sickle cell anemia. One popular and widely used DNA-editing research tool that could lead to safe and effective cures for genetic disorders is the CRISPR-Cas9 system. CRISPR-Cas9 stands for Clustered Regularly Interspaced...
52.6K
Conservative Site-specific Recombination and Phase Variation02:53

Conservative Site-specific Recombination and Phase Variation

6.1K
Because the DNA segments are cut and reorganized in a direction-specific manner, site-specific recombination has emerged as an efficient genetic engineering technique. Flippase and Cyclization recombinases or Flp and Cre, respectively, are two members of the tyrosine recombinase family derived from bacteriophages, that are used to mediate site-specific DNA insertions, deletions, and targeted expression of proteins in mammalian cell lines.
The recognition sites for Cre recombinase called LoxP...
6.1K
CRISPR/Cas9 Genome Editing01:28

CRISPR/Cas9 Genome Editing

123
The CRISPR-Cas system serves as a bacterial defense mechanism against invading genetic elements such as viruses and plasmids, forming the foundation for its adaptation as a powerful genome-editing tool. Originally discovered in prokaryotes, this system has been repurposed to revolutionize genetic engineering across a wide range of organisms, including plants, animals, and humans. The core component, Cas9, is an endonuclease derived from Streptococcus pyogenes, capable of introducing...
123

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Streamlined design and production of bioactive variants of recombinant human keratinocyte growth factor to improve acidic pH stability; promising therapeutic candidates for wound treatment.

Journal of biomolecular structure & dynamics·2026
Same author

Efficient Delivery of CRISPR-Cas9 RNP Complexes with Cyclodextrin-Based Nanosponges for Enhanced Genome Editing: TILD-CRISPR Integration.

International journal of molecular sciences·2025
Same author

CRISPR/Cas technologies in pancreatic cancer research and therapeutics: recent advances and future outlook.

Discover oncology·2025
Same author

CHO cell engineering via targeted integration of circular miR-21 decoy using CRISPR/RMCE hybrid system.

Applied microbiology and biotechnology·2024
Same author

CTXP, The Major Cobra Toxin Peptide from <i>Naja Naja Oxiana</i> Venom; A Promising Target for Antivenom Agent Development.

Current protein & peptide science·2024
Same author

Efficient site-specific integration in CHO-K1 cells using CRISPR/Cas9-modified donors.

Molecular biology reports·2023
Same journal

Developing Anti-EGFR/Anti-HER2 Bifunctional Antibody for Solid Tumors by Protein Engineering.

Biotechnology and bioengineering·2026
Same journal

Bridging Organ-on-a-Chip and Omics: A Multi-Dimensional Frontier in Biomedical Research.

Biotechnology and bioengineering·2026
Same journal

Hemopexin Purification From Human Cohn Fraction IV Paste and Its Biophysical Characterization and Functional Evaluation in Sickle Cell Disease Mice.

Biotechnology and bioengineering·2026
Same journal

Characterization and Therapeutic Potential of a Novel Lytic Phage-Derived Endolysin PA16cLys Against Uropathogenic Pseudomonas aeruginosa Biofilms.

Biotechnology and bioengineering·2026
Same journal

Modeling Multiscale Architecture of Biofilm Extracellular Matrix and Its Role in Oxygen Transport.

Biotechnology and bioengineering·2026
Same journal

A Behavior-Integrated Potency Assay for Quantitative Evaluation of Extracellular Matrix Remodeling by Mesenchymal Stem/Stromal Cells.

Biotechnology and bioengineering·2026
See all related articles

Related Experiment Video

Updated: Aug 15, 2025

Genome Editing in Mammalian Cell Lines using CRISPR-Cas
07:56

Genome Editing in Mammalian Cell Lines using CRISPR-Cas

Published on: April 11, 2019

21.9K

CRISPR-interceded CHO cell line development approaches.

Shahin Amiri1, Setare Adibzadeh1, Samaneh Ghanbari1

  • 1Department of Medical Biotechnology, Biotechnology Research Center, Pasteur Institute of Iran, Tehran, Iran.

Biotechnology and Bioengineering
|January 3, 2023
PubMed
Summary
This summary is machine-generated.

Chinese Hamster Ovary (CHO) cell line engineering using CRISPR-Cas technology enables precise genome editing. This approach enhances recombinant protein biopharmaceutical production consistency and yield by targeting safe harbor sites.

Keywords:
CRISPR-Cas9Chinese hamster ovarycell line developmentdonor designtargeted integration

More Related Videos

Generation of Genomic Deletions in Mammalian Cell Lines via CRISPR/Cas9
09:40

Generation of Genomic Deletions in Mammalian Cell Lines via CRISPR/Cas9

Published on: January 3, 2015

95.8K
Generation of Defined Genomic Modifications Using CRISPR-CAS9 in Human Pluripotent Stem Cells
09:04

Generation of Defined Genomic Modifications Using CRISPR-CAS9 in Human Pluripotent Stem Cells

Published on: September 25, 2019

8.3K

Related Experiment Videos

Last Updated: Aug 15, 2025

Genome Editing in Mammalian Cell Lines using CRISPR-Cas
07:56

Genome Editing in Mammalian Cell Lines using CRISPR-Cas

Published on: April 11, 2019

21.9K
Generation of Genomic Deletions in Mammalian Cell Lines via CRISPR/Cas9
09:40

Generation of Genomic Deletions in Mammalian Cell Lines via CRISPR/Cas9

Published on: January 3, 2015

95.8K
Generation of Defined Genomic Modifications Using CRISPR-CAS9 in Human Pluripotent Stem Cells
09:04

Generation of Defined Genomic Modifications Using CRISPR-CAS9 in Human Pluripotent Stem Cells

Published on: September 25, 2019

8.3K

Area of Science:

  • Biotechnology
  • Genomics
  • Cell Line Engineering

Background:

  • Chinese Hamster Ovary (CHO) cells are the primary host for industrial recombinant protein biopharmaceutical production.
  • CHO cells offer high-quality biologics with human-like posttranslational modifications.
  • Traditional random integration methods limit production consistency and expression levels.

Purpose of the Study:

  • To review recent advancements in CHO cell line development for biopharmaceutical production.
  • To highlight the role of targeted genome editing and CRISPR-Cas technology.
  • To discuss donor design strategies for efficient gene integration.

Main Methods:

  • CRISPR-Cas systems for targeted genome modification.
  • Identification and utilization of genomic safe harbor sites in CHO cells.
  • Development of donor DNA strategies for gene insertion.

Main Results:

  • Targeted integration ensures production consistency and high expression levels.
  • CRISPR-Cas technology allows rapid and efficient modification of CHO cell genomes.
  • Integration can be achieved at single or multiple loci based on production needs.

Conclusions:

  • Advanced CHO cell line engineering accelerates product development.
  • Targeted integration strategies improve biotherapeutic yield and quality.
  • This review provides a fundamental overview of cutting-edge CHO cell development techniques.