Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Spermatogenesis01:41

Spermatogenesis

102.9K
Spermatogenesis is the process by which haploid sperm cells are produced in the male testes. It starts with stem cells located close to the outer rim of seminiferous tubules. These spermatogonial stem cells divide asymmetrically to give rise to additional stem cells (meaning that these structures “self-renew”), as well as sperm progenitors, called spermatocytes. Importantly, this method of asymmetric mitotic division maintains a population of spermatogonial stem cells in the male...
102.9K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Clinical characteristics of Providencia rettgeri bacteraemia and antimicrobial susceptibility patterns.

Journal of infection and chemotherapy : official journal of the Japan Society of Chemotherapy·2026
Same author

Sex Differences in Characteristics and Outcomes Among Patients With Hypertrophic Cardiomyopathy: Insights From the REVEAL-HCM Study.

Journal of the American Heart Association·2026
Same author

Role of dynamic changes in human sperm heat shock protein A2 in male infertility.

The Journal of reproduction and development·2026
Same author

Cross-Species Comparison between Mouse Kidney Multi-Omics and Human Genome-Wide Association Studies Highlights Molecular Features Associated with Oxalate Nephropathy.

Kidney360·2026
Same author

Left atrial dominant cardiac sarcoidosis presenting with supraventricular tachycardia and left atrial myocardial damage: A case report.

Journal of cardiology cases·2026
Same author

Incidence, Timing, and Determinants of Stroke After Mitral Repair in Patients Without Preoperative Atrial Fibrillation.

Circulation journal : official journal of the Japanese Circulation Society·2026

Related Experiment Video

Updated: Aug 15, 2025

Preparation of Drosophila Larval and Pupal Testes for Analysis of Cell Division in Live, Intact Tissue
08:05

Preparation of Drosophila Larval and Pupal Testes for Analysis of Cell Division in Live, Intact Tissue

Published on: May 19, 2020

8.2K

Identification of active spermatogenesis using a multiphoton microscope.

Tomoki Takeda1, Shoichiro Iwatsuki1, Satoshi Nozaki1

  • 1Department of Nephro-urology, Nagoya City University Graduate School of Medical Sciences, Nagoya, Aichi, Japan.

Andrology
|January 4, 2023
PubMed
Summary

Developing objective criteria for sperm retrieval is crucial. This study introduces a novel method using multiphoton microscopy and fluorescent probes to identify spermatogenesis in testicular tissue without sectioning, improving accuracy for sperm extraction procedures.

Keywords:
F-actinectoplasmic specializationelongated spermatidmicro-TESEmultiphoton microscope

More Related Videos

A Seminiferous Tubule Squash Technique for the Cytological Analysis of Spermatogenesis Using the Mouse Model
09:40

A Seminiferous Tubule Squash Technique for the Cytological Analysis of Spermatogenesis Using the Mouse Model

Published on: February 6, 2018

15.1K
Author Spotlight: A Live Cell Imaging Technique to Study Calcium Signaling and Acrosome Exocytosis in Mouse Sperm
05:04

Author Spotlight: A Live Cell Imaging Technique to Study Calcium Signaling and Acrosome Exocytosis in Mouse Sperm

Published on: October 13, 2023

1.1K

Related Experiment Videos

Last Updated: Aug 15, 2025

Preparation of Drosophila Larval and Pupal Testes for Analysis of Cell Division in Live, Intact Tissue
08:05

Preparation of Drosophila Larval and Pupal Testes for Analysis of Cell Division in Live, Intact Tissue

Published on: May 19, 2020

8.2K
A Seminiferous Tubule Squash Technique for the Cytological Analysis of Spermatogenesis Using the Mouse Model
09:40

A Seminiferous Tubule Squash Technique for the Cytological Analysis of Spermatogenesis Using the Mouse Model

Published on: February 6, 2018

15.1K
Author Spotlight: A Live Cell Imaging Technique to Study Calcium Signaling and Acrosome Exocytosis in Mouse Sperm
05:04

Author Spotlight: A Live Cell Imaging Technique to Study Calcium Signaling and Acrosome Exocytosis in Mouse Sperm

Published on: October 13, 2023

1.1K

Area of Science:

  • Reproductive biology and urology
  • Microscopy and imaging techniques
  • Spermatogenesis research

Background:

  • Microdissection testicular sperm extraction (TESE) has variable sperm retrieval rates (25-60%).
  • Objective criteria are needed to select seminiferous tubules for spermatozoa identification.
  • Current methods may lack precision in identifying viable tubules for TESE.

Purpose of the Study:

  • To develop a non-invasive method for identifying spermatogenesis within testicular tissues.
  • To enable real-time assessment of sperm presence without tissue sectioning.
  • To enhance the selection process for microdissection TESE.

Main Methods:

  • Utilized multiphoton microscopy for observing fixed and unfixed rat testicular tissues.
  • Employed fluorescent labeling of nuclei and F-actin to visualize cellular structures.
  • Compared imaging in normal, cryptorchid, and fluorescent probe-injected testes.

Main Results:

  • Successfully identified elongated spermatids and F-actin in fixed tubules, correlating with spermatogenic stages.
  • Demonstrated that F-actin labeling and multiphoton microscopy can identify spermatogenesis in unfixed, probe-injected testes.
  • Confirmed no significant difference in cell labeling between normal and probe-injected testes, indicating low probe toxicity.

Conclusions:

  • Seminiferous epithelium and active spermatogenesis can be visualized non-invasively using fluorescent probes and multiphoton microscopy.
  • This technique allows for live imaging of testicular tissue, potentially improving sperm retrieval success rates.
  • The developed method shows promise for objective selection criteria in assisted reproductive technologies.