Safe CO₂ threshold limits for indoor long-range airborne transmission control of COVID-19

CO₂-based infection risk monitoring is highly recommended during the current COVID-19 pandemic. However, the CO₂ monitoring thresholds proposed in the literature are mainly for spaces with fixed occupants. Determining CO₂ threshold is challenging in spaces with changing occupancy due to the co-existence of quanta and CO₂ remaining from previous occupants. Here, we propose a new calculation framework for deriving safe excess CO₂ thresholds (above outdoor level), C _t, for various spaces with fixed/changing occupancy and analyze the uncertainty involved. We categorized common indoor spaces into three scenarios based on their occupancy conditions, e.g., fixed or varying infection ratios (infectors/occupants). We proved that the rebreathed fraction-based model can be applied directly for deriving C _t in the case of a fixed infection ratio (Scenario 1 and Scenario 2). In the case of varying infection ratios (Scenario 3), C _t derivation must follow the general calculation framework due to the existence of initial quanta/excess CO₂. Otherwise, C _t can be significantly biased (e.g., 260 ppm) when the infection ratio varies greatly. C _t can vary significantly based on specific space factors such as occupant number, physical activity, and community prevalence, e.g., 7 ppm for gym and 890 ppm for lecture hall, indicating C _t must be determined on a case-by-case basis. An uncertainty of up to 6 orders of magnitude for C _t was found for all cases due to uncertainty in emissions of quanta and CO₂, thus emphasizing the role of accurate emissions data in determining C _t.