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Silencing circUSP48 suppresses osteosarcoma progression by regulating the miR-335/ smad nuclear interacting protein 1

Yue Luo1, Bo Yang1, Xiaopin Yuan1

  • 1Department of Orthopedics, Renmin Hospital of Wuhan University, Wuhan, China.

Journal of Clinical Laboratory Analysis
|January 4, 2023
PubMed
Abstract

Insights

Circular USP 48 (circUSP48) promotes osteosarcoma (OS) progression by sponging miR-335 to increase SNIP1. Silencing circUSP48 inhibits OS cell proliferation, invasion, migration, and tumor growth, suggesting circUSP48 as a potential therapeutic target.

Area of Science:

  • Oncology
  • Molecular Biology
  • Genetics

Background:

  • Circular RNAs (circRNAs) play roles in osteosarcoma (OS) development.
  • The specific role of circUSP48 in OS progression is currently unknown.

Purpose of the Study:

  • To investigate the function and mechanism of circUSP48 in osteosarcoma.
  • To determine if circUSP48 acts as an oncogene in OS.

Main Methods:

  • Quantitative real-time PCR (qRT-PCR) to assess expression levels of circUSP48, miR-335, and SNIP1.
  • circUSP48 validation using Sanger sequencing, RNase R treatment, and FISH assays.
  • Loss-of-function experiments to evaluate circUSP48's role in OS cell lines and in vivo tumor growth.

Main Results:

  • Silencing circUSP48 suppressed proliferation, invasion, and migration of OS cells in vitro.
  • circUSP48 inhibition also reduced tumor growth in vivo.
  • circUSP48 promotes OS malignancy by sponging miR-335, leading to SNIP1 upregulation.

Conclusions:

  • circUSP48 functions as an oncogene in osteosarcoma.
  • circUSP48 may represent a novel therapeutic target for osteosarcoma treatment.
Keywords:
SNIP1circUSP48miR-335osteosarcomaprogression

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