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Peripheral thermosensation is the perception of external temperature. A change in temperature (on the surface of the skin and other tissues) is detected by a family of temperature-sensitive ion channels called Transient Receptor Potential, or TRP, receptors. These receptors are located on free nerve endings. Those detecting cold temperatures are closer to the surface of the skin than the nerve endings detecting warmth. These thermoTRP channels, while temperature selective, have relatively...
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Body temperature can be assessed using various devices and measured in Celsius or Fahrenheit.
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Assessing tympanic membrane temperature involves using a tympanic membrane thermometer (TMT). Here is a step-by-step guide:
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Thermal Measurement Techniques in Analytical Microfluidic Devices
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Single-cell thermometry with a nanothermocouple probe.

Li-Qiu Huang1, Xin-Lei Ding1, Xiao-Tong Pan1

  • 1State Key Laboratory of Analytical Chemistry for Life Science, School of Chemistry and Chemical Engineering, Nanjing University, Nanjing 210023, China. zhongqiuli@nju.edu.cn.

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A new nanopipette thermocouple probe enables precise single-cell temperature sensing. This technology reveals metabolic differences and temperature variations in cells, aiding drug screening and disease diagnosis.

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Area of Science:

  • Biotechnology
  • Cell Biology
  • Nanotechnology

Background:

  • Cellular metabolism influences intracellular temperature.
  • Accurate single-cell temperature measurement is crucial for understanding cellular processes.
  • Existing methods lack the resolution and speed for dynamic single-cell analysis.

Purpose of the Study:

  • To develop a nanopipette-based thermocouple probe for high-resolution intracellular temperature sensing.
  • To investigate metabolic differences between cell types using temperature profiles.
  • To evaluate the potential of this technology for drug screening and disease diagnosis.

Main Methods:

  • Construction of a nanopipette-based thermocouple probe.
  • Application of the probe for single-cell temperature measurements.
  • Analysis of temperature responses in diverse cell types and 3D cell cultures.
  • Assessment of drug effects on cellular temperature.

Main Results:

  • The probe demonstrated high temperature resolution, rapid response, and stability.
  • Distinct intracellular temperatures were observed in different cell types, correlating with metabolism.
  • Cellular temperature responses to various drugs were successfully measured.
  • Inner temperature gradients were detected in three-dimensional cell culture models.

Conclusions:

  • The nanopipette thermocouple probe is a powerful tool for single-cell thermometry.
  • Intracellular temperature mapping can reveal metabolic states and drug responses.
  • This technology offers potential for advancing drug screening and disease diagnostics.