Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

The de Broglie Wavelength02:32

The de Broglie Wavelength

26.1K
In the macroscopic world, objects that are large enough to be seen by the naked eye follow the rules of classical physics. A billiard ball moving on a table will behave like a particle; it will continue traveling in a straight line unless it collides with another ball, or it is acted on by some other force, such as friction. The ball has a well-defined position and velocity or well-defined momentum, p = mv, which is defined by mass m and velocity v at any given moment. This is the typical...
26.1K
Electron Microscope Tomography and Single-particle Reconstruction01:07

Electron Microscope Tomography and Single-particle Reconstruction

2.5K
Transmission electron microscopy (TEM) can be used to determine the 3D structure of biological samples with the help of techniques such as electron microscope tomography and single-particle reconstruction. While single-particle reconstruction can examine macromolecules and macromolecular complexes in vitro conditions only, tomography permits the study of cell components or small cells in vivo.
Electron Tomography
Electron tomography can be performed either in TEM or STEM (scanning transmission...
2.5K
X-ray Crystallography02:18

X-ray Crystallography

24.1K
The size of the unit cell and the arrangement of atoms in a crystal may be determined from measurements of the diffraction of X-rays by the crystal, termed X-ray crystallography.
Diffraction
Diffraction is the change in the direction of travel experienced by an electromagnetic wave when it encounters a physical barrier whose dimensions are comparable to those of the wavelength of the light. X-rays are electromagnetic radiation with wavelengths about as long as the distance between neighboring...
24.1K
X-ray Diffraction of Biological Samples01:10

X-ray Diffraction of Biological Samples

3.9K
X-ray diffraction or XRD is an analytical tool that utilizes X-rays to study ordered structures such as crystalline organic and inorganic samples, polycrystalline materials, proteins, carbohydrates, and drugs.
According to Bragg's law, when X-rays strike the sample positioned on a stage, the rays are  scattered by the electron clouds around the sample atoms. The  X-ray diffraction or scattering is caused by constructive interference of the X-ray waves that reflect off the internal...
3.9K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Optical and Electron Transparent Polycrystalline Boron Doped Diamond Membranes for Nanoscale Correlative Structure-Electrochemical Measurements.

ACS nano·2025
Same author

Ferroelastic writing of crystal directions in oxide thin films.

Nature nanotechnology·2025
Same author

Toward a Procedure for the Template Free Growth of Te Nanowires Across an Insulator by Electrodeposition.

The journal of physical chemistry. C, Nanomaterials and interfaces·2024
Same author

Superstructure reflections in tilted perovskites.

Acta crystallographica. Section A, Foundations and advances·2024
Same author

An ultra high-endurance memristor using back-end-of-line amorphous SiC.

Scientific reports·2024
Same author

3D electron diffraction goes multipolar.

IUCrJ·2024
Same journal

Towards light-coupled sample preparation for time-resolved cryoEM studies.

IUCrJ·2026
Same journal

Cryo-EM analysis of cooperative conformational changes in the SARS-CoV-2 spike protein trimer.

IUCrJ·2026
Same journal

Towards time-resolved MicroED grid preparation using mix-and-inject gas dynamic virtual nozzles.

IUCrJ·2026
Same journal

How cryoEM has advanced our understanding of bacteriophages and bacteriocins targeting Clostridioides difficile.

IUCrJ·2026
Same journal

CryoEM structures reveal allosteric regulation of the catalytic activity of the multi-protein human MAT enzyme complexes.

IUCrJ·2026
Same journal

Cryo-EM-guided subtractive optimization of a novel VCP/p97 inhibitor.

IUCrJ·2026
See all related articles

Related Experiment Video

Updated: Aug 15, 2025

Study of Protein Dynamics via Neutron Spin Echo Spectroscopy
08:03

Study of Protein Dynamics via Neutron Spin Echo Spectroscopy

Published on: April 13, 2022

2.2K

Modelling fine-sliced three dimensional electron diffraction data with dynamical Bloch-wave simulations.

Anton Cleverley1, Richard Beanland2

  • 1Department of Chemistry, University of Warwick, Coventry CV4 7AL, United Kingdom.

Iucrj
|January 4, 2023
PubMed
Summary
This summary is machine-generated.

Electron diffraction (ED) structure refinement is improved using dynamical Bloch-wave simulations. This method enhances accuracy for sub-micrometre crystals, outperforming traditional kinematic models in electron diffraction data processing.

Keywords:
3D electron diffraction3D-EDcomputational modellingdynamical refinementdynamical simulations

More Related Videos

High-Resolution Neutron Spectroscopy to Study Picosecond-Nanosecond Dynamics of Proteins and Hydration Water
08:48

High-Resolution Neutron Spectroscopy to Study Picosecond-Nanosecond Dynamics of Proteins and Hydration Water

Published on: April 28, 2022

1.8K
Studying Soft-matter and Biological Systems over a Wide Length-scale from Nanometer and Micrometer Sizes at the Small-angle Neutron Diffractometer KWS-2
11:27

Studying Soft-matter and Biological Systems over a Wide Length-scale from Nanometer and Micrometer Sizes at the Small-angle Neutron Diffractometer KWS-2

Published on: December 8, 2016

12.3K

Related Experiment Videos

Last Updated: Aug 15, 2025

Study of Protein Dynamics via Neutron Spin Echo Spectroscopy
08:03

Study of Protein Dynamics via Neutron Spin Echo Spectroscopy

Published on: April 13, 2022

2.2K
High-Resolution Neutron Spectroscopy to Study Picosecond-Nanosecond Dynamics of Proteins and Hydration Water
08:48

High-Resolution Neutron Spectroscopy to Study Picosecond-Nanosecond Dynamics of Proteins and Hydration Water

Published on: April 28, 2022

1.8K
Studying Soft-matter and Biological Systems over a Wide Length-scale from Nanometer and Micrometer Sizes at the Small-angle Neutron Diffractometer KWS-2
11:27

Studying Soft-matter and Biological Systems over a Wide Length-scale from Nanometer and Micrometer Sizes at the Small-angle Neutron Diffractometer KWS-2

Published on: December 8, 2016

12.3K

Area of Science:

  • Materials Science
  • Crystallography
  • Electron Microscopy

Background:

  • Electron diffraction (ED) offers advantages for analyzing small crystals and light elements.
  • Current ED data processing often uses X-ray diffraction software and kinematic theory, which doesn't fully account for ED's unique physics.
  • This discrepancy limits the accuracy of structure solution and refinement in ED.

Purpose of the Study:

  • To develop and validate a more accurate method for processing continuous-rotation electron diffraction data.
  • To improve the refinement of crystal structures using ED, especially for sub-micrometre crystals.
  • To address limitations of current kinematic models in ED data analysis.

Main Methods:

  • Dynamical Bloch-wave simulations were employed to model ED data with high angular resolution (∼0.1°).
  • A novel method was introduced for optimizing crystal orientation, considering incident beam angular range and slew rate.
  • Rocking curves were used for accurate comparisons with simulated data for a silicon lamella.

Main Results:

  • Dynamical simulations significantly improved the accuracy of ED data modeling compared to kinematic methods.
  • The R1 factor was reduced from 26% (kinematic) to 6.8% (dynamical simulations).
  • The proposed method accurately accounts for experimental parameters like crystal orientation and beam slew rate.

Conclusions:

  • Dynamical Bloch-wave simulations provide a more accurate approach for refining structures from ED data.
  • This advancement is crucial for unlocking the full potential of ED for small crystals and light elements.
  • The findings pave the way for more reliable structure determination using electron diffraction.