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Updated: Aug 15, 2025
MS2-Affinity Purification Coupled with RNA Sequencing in Gram-Positive Bacteria
Published on: February 23, 2021
siRNAs pools generated in Escherichia coli exhibit strong RNA-interference activity against influenza virus genomic
Riccardo Villa1, Sabrina Renzi1, Silvia Dotti1
1Istituto Zooprofilattico Sperimentale della Lombardia e dell'Emilia Romagna (IZSLER), Laboratorio di Controllo di Prodotti Biologici, Farmaceutici e Convalida di Processi Produttivi, via A. Bianchi 9, Brescia, Italy.
Abstract:
Due to the recurrent pandemic outbreaks that occurred during the last century, Influenza A viruses are considered a serious potential danger to human health. Among the innate immune pathways in eukaryotes, RNA interference plays a significant role in the interaction between viruses and host cells. RNA interference is addressed by small dsRNA molecules produced by the host itself (miRNAs, i.e. "micro-RNAs") but can be triggered also by the administration of exogenous short RNAs (siRNAs, "short interfering RNAs"). In this work, artificial siRNA pools targeting NP and PB genomic regions of the Influenza virus were produced in engineered Escherichia coli, adapting a published protocol. In a MDCK cell in vitro model, these preparations were challenged against reporter vectors bearing viral genomic sequences. A strong and specific RNA interference activity was observed, and the details of this action were indagated.
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