Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Overview of Exosomes01:36

Overview of Exosomes

2.8K
Exosomes are stable, lipid bilayer-enclosed vesicles capable of crossing biological barriers. They can carry a wide range of molecules required for intercellular communication. Once exosomes are released from the cell where they originated, they enter a recipient cell through various pathways such as fusion, receptor-mediated endocytosis, macropinocytosis, and phagocytosis.
Stahl et al. discovered exosomes in 1983, but the exosomes were initially considered waste products released from the...
2.8K
Overview of Secretory Vesicles01:33

Overview of Secretory Vesicles

8.6K
Secretory vesicles, also known as dense core vesicles (DCVs), are membrane-bound vesicles that transport secretory proteins, such as hormones or neurotransmitters. Regulated secretory vesicles transport proteins from the trans-Golgi network to the exterior of the cell. Proteins present in regulated secretory vesicles are required to be rapidly exocytosed in large amounts upon a specific stimulus.
Various proteins regulate the aggregation of molecules inside the secretory vesicles. Chromogranins...
8.6K
Intralumenal Vesicles and Multivesicular Bodies01:38

Intralumenal Vesicles and Multivesicular Bodies

3.6K
Intraluminal vesicles (ILVs) are small vesicles 50-80 nm in diameter formed during the maturation of early endosomes. A specialized endosome containing numerous ILVs is called a multivesicular body (MVB). ILVs contain internalized molecules such as antigens, nucleic acids, proteins, and metabolites. Some of these molecules are released from the MVBs inside exosomes and are transported to other cells. Other MVBs contain molecules that are retained in the ILVs and are later degraded within the...
3.6K
The Effect of Aging on Tissues01:19

The Effect of Aging on Tissues

2.2K
Several body functions deteriorate with age. The external signs of aging are easily identifiable. For example, the skin becomes dry, less elastic, and thins out, forming wrinkles. The skin of the face begins to appear looser due to a decrease in the levels of elastic and collagen fibers in the connective tissue. Additionally, melanin production in the hair follicle decreases with age, resulting in gray hair. Moreover, the senses of sight and hearing decline, so glasses and hearing aids may...
2.2K
Maturation of Endosomes01:28

Maturation of Endosomes

4.3K
The early endosome containing internalized molecules matures through transformations in its location, morphology, intraluminal pH, and membrane protein composition. Together, these changes result in a more acidic late endosome that contains multiple intraluminal vesicles; therefore, the late endosome is also called a multivesicular body (MVB).
Changes in location
The maturing endosome moves along microtubules from the periphery of the cell towards the perinuclear region. This movement of the...
4.3K
Receptor Downregulation in MVBs01:15

Receptor Downregulation in MVBs

2.1K
Multivesicular bodies (MVBs) are mature endosomes that sort ubiquitinated proteins and then fuse with lysosomes to degrade the sorted proteins. Epidermal growth factor (EGF) and its receptor (EGFR) form a complex that can be internalized through endocytosis, sorted into an MVB, and later degraded.
The EGFR can initiate signaling pathways that  lead to cell proliferation, migration, and differentiation. Overexpression of EGFR  stimulates cells to proliferate. Excessive  EGFR...
2.1K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Acute brain dysfunction clusters in COVID-19: a pilot machine learning-based analysis of the COVID-D cohort.

Intensive care medicine experimental·2026
Same author

High inappropriate red blood cell transfusion rate despite low overall use: a real-world multicenter study in 43 Spanish hospitals.

Frontiers in medicine·2026
Same author

Postoperative delirium and the limits of EEG monitoring without goal-directed anesthetic management.

Minerva anestesiologica·2026
Same author

Chemokines and redox homeostasis in aging: A dynamic relationship.

Mechanisms of ageing and development·2026
Same author

Association of mortality and neurological outcomes with hypocapnia in adult patients with acute brain injury: an updated meta-analysis.

Critical care (London, England)·2026
Same author

Peripheral blood biomarkers RCAN1, Clusterin, RAGE, and malondialdehyde for early diagnosis and progression of Alzheimer's disease.

BMC medicine·2026

Related Experiment Video

Updated: Aug 15, 2025

Evaluation of the Storage Stability of Extracellular Vesicles
11:31

Evaluation of the Storage Stability of Extracellular Vesicles

Published on: May 22, 2019

14.3K

Extracellular Vesicles and Cellular Ageing.

Nekane Romero-García1, Cristina Mas-Bargues1, Javier Huete-Acevedo1

  • 1Freshage Research Group, Department of Physiology, Faculty of Medicine, University of Valencia, Centro de Investigación Biomédica en Red Fragilidad y Envejecimiento Saludable-Instituto de Salud Carlos III (CIBERFES-ISCIII), INCLIVA, Valencia, Spain.

Sub-Cellular Biochemistry
|January 4, 2023
PubMed
Summary
This summary is machine-generated.

Stem cell therapies show promise for regenerative medicine in aging. Extracellular vesicles (EVs), released by cells, are being explored as cell-free therapies to combat aging hallmarks and age-related diseases.

Keywords:
Age-related diseasesAgeingExtracellular vesiclesIntercellular communicationRegenerative medicineStem cells

More Related Videos

Isolation and Analysis of Traceable and Functionalized Extracellular Vesicles from the Plasma and Solid Tissues
09:57

Isolation and Analysis of Traceable and Functionalized Extracellular Vesicles from the Plasma and Solid Tissues

Published on: October 17, 2022

2.2K
Enrichment of Astrocyte-Derived Extracellular Vesicles from Human Plasma
07:34

Enrichment of Astrocyte-Derived Extracellular Vesicles from Human Plasma

Published on: August 3, 2022

3.0K

Related Experiment Videos

Last Updated: Aug 15, 2025

Evaluation of the Storage Stability of Extracellular Vesicles
11:31

Evaluation of the Storage Stability of Extracellular Vesicles

Published on: May 22, 2019

14.3K
Isolation and Analysis of Traceable and Functionalized Extracellular Vesicles from the Plasma and Solid Tissues
09:57

Isolation and Analysis of Traceable and Functionalized Extracellular Vesicles from the Plasma and Solid Tissues

Published on: October 17, 2022

2.2K
Enrichment of Astrocyte-Derived Extracellular Vesicles from Human Plasma
07:34

Enrichment of Astrocyte-Derived Extracellular Vesicles from Human Plasma

Published on: August 3, 2022

3.0K

Area of Science:

  • Regenerative Medicine
  • Cell Biology
  • Gerontology

Background:

  • Aging is a complex process involving cellular dysfunction and organ degeneration.
  • Stem cell therapies offer potential for tissue repair and functional recovery in aging.
  • Altered cell-cell communication contributes to aging and age-related diseases.

Purpose of the Study:

  • To explore the role of microenvironment components, particularly extracellular vesicles (EVs), in aging.
  • To investigate the therapeutic potential of EVs in regenerative medicine for aging.
  • To assess EVs as biomarkers and cell-free therapies for age-related conditions.

Main Methods:

  • Review of current research on stem cell therapies and aging.
  • Analysis of the composition and function of extracellular vesicles (EVs).
  • Evaluation of preclinical and clinical studies on stem cell engraftments and EV-based therapies.

Main Results:

  • Stem cells possess self-renewal, differentiation, and immunomodulation properties suitable for regenerative medicine.
  • Extracellular vesicles (EVs) carry bioactive molecules that can modify recipient cell function and fate.
  • EVs show potential as biomarkers for diseases and as cell-free therapeutic agents for aging.

Conclusions:

  • Stem cell-based therapies are under investigation for treating age-related diseases.
  • Extracellular vesicles (EVs) represent a promising avenue for cell-free therapies to address aging hallmarks.
  • Further research into EVs could lead to next-generation treatments for age-related conditions.