Related Experiment Video
Updated: Aug 15, 2025

A High Throughput Screen for Biomining Cellulase Activity from Metagenomic Libraries
Published on: February 1, 2011
Isolation and Characterization of Bacteria from Natural Hot Spring and Insights into the Thermophilic Cellulase
Indira Sarangthem1, Lynda Rajkumari2, Ng Ngashangva2
1Institute of Bioresources and Sustainable Development, Imphal, Manipur, 795001, India. sidevi1@yahoo.co.in.
Abstract:
A thermophilic cellulase-producing bacterium, Bacillus velezensis strain MRC 5958, from Bakra natural hot Springs, India was characterized through genome sequencing. It has a genome size of 4,467,129 bp and a GC content of 45.7%. A cellulase purified from its fermentation broth has a molecular weight of about 18 kDa. The optimum temperature and pH for carboxymethyl cellulase activity were at 55 °C and pH ~ 7.0. The enzyme is stable over a wide range of temperatures from 30 °C to 70 °C with maximum activity observed at 48 h of incubation. The strain produces cellulase on alkali-treated sugarcane bagasse, rice straw, rice husk, rice bran, and sawdust. The sugarcane bagasse exhibited the most effective carbon source for cellulase production at (85 U/ml) followed by rice bran (68 U/ml), rice husk (60 U/ml), rice straw (48 U/ml), and sawdust (39 U/ml). Therefore, this strain can be a potential thermostable cellulase-producing candidate for converting the waste biomass into biofuel and other industrial enzymes.
More Related Videos
Related Concept Videos
Hyperthermophilic Bacteria
Diversity of Archaea I
Techniques for Isolation of Pure Cultures
Anoxygenic Phototrophic Bacteria
Diversity of Archaea III

