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Updated: Aug 15, 2025

Generic Protocol for Optimization of Heterologous Protein Production Using Automated Microbioreactor Technology
Published on: December 15, 2017
Technological interventions in improving the functionality of proteins during processing of meat analogs
Pavan Kumar1,2, Neelesh Sharma3, Muideen Adewale Ahmed1
1Institute of Tropical Agriculture and Food Security, Universiti Putra Malaysia (UPM), Seri Kembangan, Malaysia.
Exploring sustainable meat alternatives, this review details various proteins and processing techniques crucial for creating effective meat analogs. Key protein sources and innovative manufacturing methods are discussed for improved texture and functionality.
Area of Science:
- Food Science and Technology
- Sustainable Food Production
- Protein Chemistry
Background:
- Meat analogs offer a sustainable alternative to conventional meat products.
- Proteins are essential for mimicking the texture, nutritional value, and sensory attributes of meat.
- Sourcing and processing diverse proteins for meat analogs present significant challenges.
Purpose of the Study:
- To review and discuss various protein sources suitable for meat analog production.
- To explore technological interventions for enhancing protein functionality in meat analogs.
- To provide a comprehensive overview of current research in meat analog protein development.
Main Methods:
- Literature review of scientific papers and industry reports.
- Analysis of protein functionalities from diverse sources (e.g., soy, wheat, algae, mycoproteins).
- Examination of processing techniques like extrusion, 3D printing, and freeze structuring.
Main Results:
- Identified soy proteins, wheat gluten, zein, algal proteins, mycoproteins, pulses, potato, oilseeds, pseudo-cereals, and grass as potential protein sources.
- Highlighted extrusion, spinning, Couette shear cell, additive manufacturing, and freeze structuring as key processing interventions.
- Demonstrated the impact of protein source and processing on meat analog characteristics.
Conclusions:
- A wide array of plant-based, algal, and fungal proteins can be utilized for meat analog development.
- Advanced processing technologies are vital for optimizing the texture and functionality of meat analogs.
- Further research into novel protein sources and processing methods is essential for advancing sustainable meat alternatives.
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