The enrichment characterisation of Nitrospira under high DO conditions

Nitrite-oxidizing bacteria (NOB) are crucial to nitrification and nitrogen elimination in wastewater treatment. Mass reports exist on the links between NOB and other microorganisms, for instance, ammonia-oxidizing bacteria (AOB). However, a few studies exist on the enrichment characterisation of NOB under high dissolved oxygen (DO) conditions. In this study, NOB was designed to be enriched individually under high DO conditions in a continuous aeration sequencing batch reactor (SBR), and the kinetic characterisation of NOB was evaluated. The analysis revealed that the average NO₂^--N removal rate was steady above 98%, with DO and NO₂^--N being 3-5 mg L^-1 and 50-450 mg L^-1, respectively. The NO₂^--N removal efficiency of the system was significantly enhanced and better than in other studies. The high-throughput sequencing suggested that Parcubacteria_ genera_incertae_sedis was the first dominant genus (21.99%), which often appeared in the NOB biological community with Nitrospira. However, the dominant genus NOB was Nitrospira rather than Nitrobacter (8.49%). This result suggested that Nitrospira was capable of higher NO₂^--N removal. But lower relative abundance indicated that excessive NO₂^--N had an adverse effect on the enrichment and activity of Nitrospira. In addition, the nitrite half-saturation constant (K) and the oxygen half-saturation constant (K) were 1.71 ± 0.19 mg L^-1 and 0.95 ± 0.10 mg L^-1, respectively. These results showed that the enriched Nitrospira bacteria had different characteristics at the strain level, which can be used as a theoretical basis for wastewater treatment plant design and optimisation.