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Assessing wound closure in mice using skin-punch biopsy
Mary B Rowland1, Patrick E Moore1, Chuong Bui2
1Department of Biological Sciences, University of Alabama, Tuscaloosa, AL 35401, USA.
STAR Protocols
|January 5, 2023
Summary
Screening mouse models for wound healing defects is simplified with a new skin-punch biopsy method. This approach efficiently identifies issues in myofibroblast function, aiding further research into tissue repair.
Area of Science:
- Wound healing research
- Myofibroblast biology
- Animal model development
Background:
- Myofibroblast dysfunction is implicated in various tissue healing defects.
- Accurate screening methods are crucial for understanding these defects.
- Existing methods may require specialized training or equipment.
Purpose of the Study:
- To introduce a simplified skin-punch biopsy technique for screening mouse models.
- To assess the efficacy of this method in identifying wound closure defects.
- To provide a proof of concept for investigating myofibroblast function in wound healing.
Main Methods:
- Utilized a straightforward skin-punch biopsy procedure.
- Applied the method to screen mouse models for impaired wound closure.
- Focused on evaluating myofibroblast function's role in skin wound healing.
Main Results:
- The skin-punch biopsy approach is simple and does not require extensive surgical expertise.
- The method serves as an effective initial screen for identifying potential wound healing defects.
- Results can guide decisions on further, more in-depth investigations.
Conclusions:
- A simple skin-punch biopsy method can effectively screen mouse models for wound healing defects.
- This technique facilitates the study of myofibroblast function in skin wound repair.
- The approach offers a cost-effective and accessible tool for researchers in tissue regeneration.
