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RNA Pull-down Procedure to Identify RNA Targets of a Long Non-coding RNA
Published on: April 10, 2018
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Long non-coding RNA DNMBP-AS1 promotes prostate cancer development by regulating LCLAT1
Xiangang Yin1, Suying Wang1, Rong Ge1
1Department of Diagnosis, Ningbo Diagnostic Pathology Center, Ningbo, China.
Systems Biology in Reproductive Medicine
|January 5, 2023
Summary
Long non-coding RNA DNMBP antisense RNA 1 (DNMBP-AS1) promotes prostate cancer (PCa) progression by enhancing lysocardiolipin acyltransferase 1 (LCLAT1) expression. This study reveals DNMBP-AS1 as a potential driver for PCa development.
Area of Science:
- Oncology
- Molecular Biology
- Genetics
Background:
- Prostate cancer (PCa) poses a significant global health challenge for men.
- Long non-coding RNAs (lncRNAs) play crucial roles in various human cancers.
- The specific function of lncRNA DNMBP antisense RNA 1 (DNMBP-AS1) in PCa remains largely unexplored.
Purpose of the Study:
- To investigate the role and mechanism of DNMBP-AS1 in prostate cancer.
- To determine if DNMBP-AS1 influences PCa cell proliferation, migration, and invasion.
- To elucidate the molecular pathways regulated by DNMBP-AS1 in PCa.
Main Methods:
- Gene expression analysis using RT-qPCR.
- Cellular function assays including CCK-8, colony formation, EdU staining, and Transwell assays.
- Molecular mechanism studies utilizing RNA pull-down, RIP, and luciferase reporter assays.
Main Results:
- DNMBP-AS1 expression was significantly upregulated in PCa cell lines.
- Knockdown of DNMBP-AS1 inhibited PCa cell proliferation, migration, and invasion.
- DNMBP-AS1 was found to sponge microRNA-6766-3p (miR-6766-3p), thereby regulating LCLAT1 expression.
- DNMBP-AS1 stabilizes LCLAT1 expression by recruiting ELAVL1.
- Rescue assays confirmed that DNMBP-AS1 promotes PCa progression via LCLAT1 upregulation.
Conclusions:
- DNMBP-AS1 acts as an oncogenic lncRNA in prostate cancer.
- DNMBP-AS1 promotes PCa cell proliferation, migration, and invasion by sponging miR-6766-3p and enhancing LCLAT1 expression.
- DNMBP-AS1 represents a potential therapeutic target and biomarker for prostate cancer.
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