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Updated: Aug 15, 2025

A Simple Method for Isolation of Soybean Protoplasts and Application to Transient Gene Expression Analyses
Published on: January 25, 2018
Soybean seed protein storage vacuoles for expression of recombinant molecules
G R Vianna1, N B Cunha2, E L Rech1
1Embrapa Genetic Resources and Biotechnology/National Institute of Science and Technology in Synthetic Biology, Brasília, Distrito Federal, 70770-917, Brazil.
Abstract:
Soybean is one of the most important protein sources for human consumption and livestock feed. Soy production also allows the biosynthesis of edible oils, biodiesel, and biofertilizers. With the advent of modern agricultural biotechnology, soybean plants have also converted into bioreactors of therapeutic proteins and industrial enzymes. Soybean's characteristics, such as protein storage vacuoles (PSVs) and other unique organelles, allow the plant to be exploited as an accumulator of heterologous proteins under high stability and scalability conditions, and that maintains its basic functions. This review reports the main aspects of heterologous protein accumulation in soybean PSVs.
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