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Published on: June 4, 2020
Continuous chromogenic tissue factor assay: comparison to clot-based assays and sensitivity established using pure
1Department of Pathology, University of Colorado, School of Medicine, Denver 80262.
Abstract:
The continuous chromogenic tissue factor assay has been compared to one-stage and two-stage coagulation assays, and its sensitivity has been established using purified human placental tissue factor. Log-log plots of the clot-times versus A405/min2 are linear over the mutually useful ranges of tissue factor concentration. Using human placental tissue factor reconstituted into phosphatidylserine-phosphatidylcholine (30:70) vesicles, the chromogenic assay quantitatively detected tissue factor in dilutions containing from 3 femtomoles to less than 10 attomoles of protein.
