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Guide RNA library-based CRISPR screens in plants: opportunities and challenges
Changtian Pan1, Gen Li1, Anindya Bandyopadhyay2
1Department of Plant Science and Landscape Architecture, University of Maryland, College Park, MD 20742, USA.
Current Opinion in Biotechnology
|January 5, 2023
Summary
CRISPR-Cas technology enables high-throughput genetic screening in plants using guide RNA libraries. This review assesses current methods, delivery techniques, and future directions for plant genomic research.
Area of Science:
- Plant genomics
- Functional genomics
- Biotechnology
Background:
- Next-generation sequencing advanced genome and transcriptome analysis.
- Large-scale genetic screening in plants remains a significant challenge.
- CRISPR-Cas nucleases offer a promising solution for plant functional genomics.
Purpose of the Study:
- To review recent advancements in guide RNA (gRNA) library-based CRISPR screens in plants.
- To critically assess current CRISPR screening approaches and delivery methods.
- To highlight challenges and future perspectives for CRISPR screens in plants.
Main Methods:
- Review of current literature on CRISPR-Cas systems in plants.
- Analysis of guide RNA library construction and application.
- Evaluation of various delivery methods for CRISPR components in plant cells.
- Discussion of functional genomic screening strategies using CRISPR technology.
Main Results:
- CRISPR-Cas systems combined with gRNA libraries show potential for high-throughput plant genetic screening.
- Progress has been made in optimizing CRISPR screens for directed evolution and trait engineering.
- Various delivery methods are being explored to enhance CRISPR efficiency in plants.
- The technology is still developing, with ongoing challenges in efficiency and application.
Conclusions:
- CRISPR-Cas technology, particularly with gRNA libraries, holds significant promise for advancing plant functional genomics.
- Further research and development are needed to overcome current limitations and fully realize the potential of CRISPR screens in plants.
- This review provides a critical overview and outlook for future applications in plant science.
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