CdSe-Co₃O₄@TiO₂ nanoflower-based photoelectrochemical platform probing visible light-driven virus detection

The design and construction of a visible light-driven photoelectrochemical (PEC) device is described based on a CdSe-Co₃O₄@TiO₂ nanoflower (NF). Moreover, an application to the ultrasensitive detection of viruses, such as hepatitis E virus (HEV), HEV-like particles (HEV-LPs), and SARS-CoV-2 spike protein in complicated lysate solution, is demonstrated. The photocurrent response output of a PEC device based on CdSe-Co₃O₄@TiO₂ is enhanced compared with the individual components, TiO₂ and CdSe-Co₃O₄. This can be attributed to the CdSe quantum dot (QD) sensitization effect and strong visible light absorption to improve overall system stability. A robust oxygen-evolving catalyst (Co₃O₄) coupled at the hole-trapping site (CdSe) extends the interfacial carrier lifetime, and the energy conversion efficiency was improved. The effective hybridization between the antibody and virus resulted in a linear relationship between the change in photocurrent density and the HEV-LP concentration ranging from 10 fg mL^-1 to 10 ng mL^-1, with a detection limit of 3.5 fg mL^-1. This CdSe-Co₃O₄@TiO₂-based PEC device achieved considerable sensitivity, good specificity, and acceptable stability and demonstrated a significant ability to develop an upgraded device with affordable and portable biosensing capabilities.