Related Experiment Video
Updated: Aug 15, 2025
09:08
Mapping Genome-wide Accessible Chromatin in Primary Human T Lymphocytes by ATAC-Seq
Published on: November 13, 2017
18.1K
Solid-phase capture and profiling of open chromatin by spatial ATAC
Enric Llorens-Bobadilla1, Margherita Zamboni2,3, Maja Marklund3
1Department of Cell and Molecular Biology, Karolinska Institute, Stockholm, Sweden. enric.llorens@ki.se.
Nature Biotechnology
|January 5, 2023
Summary
Spatial ATAC profiling provides genome-wide epigenomic data with spatial resolution. This new method reveals regulatory programs in tissue development and human diseases.
Area of Science:
- Epigenomics
- Spatial Biology
- Genomics
Background:
- Current epigenomic profiling methods lack spatial resolution.
- Understanding genome-wide epigenetic regulation in specific tissue locations is challenging.
Purpose of the Study:
- To introduce a novel method for spatially resolved epigenomics.
- To enable the discovery of regulatory programs linked to spatial gene expression.
Main Methods:
- Integration of transposase-accessible chromatin profiling in tissue sections.
- Utilizing barcoded solid-phase capture for spatial epigenomic analysis.
Main Results:
- Demonstrated the capability of spatial ATAC for epigenomic profiling with spatial resolution.
- Enabled discovery of regulatory programs in mouse organogenesis and lineage differentiation.
- Applied to analyze epigenomic alterations in human pathology.
Conclusions:
- Spatial ATAC is a powerful tool for spatially resolved epigenomics.
- This method advances the understanding of gene regulation in complex biological systems.
- Opens new avenues for studying tissue development and disease.
