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Chromatin Immunoprecipitation- ChIP

Chromatin Immunoprecipitation- ChIP

Chromatin immunoprecipitation, or ChIP, is an antibody-based technique used to identify sites on DNA that bind to transcription factors of interest or histone proteins. It also helps determine the type of histone modifications such as acetylation, phosphorylation, or methylation.
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ChIP can be divided into two types - X-ChIP and N-ChIP. X-ChIP involves in vivo cross-linking of histones and regulatory proteins to DNA, fragmenting the DNA by sonication, and isolating the protein-DNA...

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Updated: Aug 15, 2025

Mapping Genome-wide Accessible Chromatin in Primary Human T Lymphocytes by ATAC-Seq

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Published on: November 13, 2017

Solid-phase capture and profiling of open chromatin by spatial ATAC.

Enric Llorens-Bobadilla¹, Margherita Zamboni^2,3, Maja Marklund³

¹Department of Cell and Molecular Biology, Karolinska Institute, Stockholm, Sweden. enric.llorens@ki.se.

Nature Biotechnology

|January 5, 2023

Summary

Spatial ATAC profiling provides genome-wide epigenomic data with spatial resolution. This new method reveals regulatory programs in tissue development and human diseases.

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Mapping Genome-wide Accessible Chromatin in Primary Human T Lymphocytes by ATAC-Seq

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ATAC-Seq Optimization for Cancer Epigenetics Research

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Area of Science:

Epigenomics
Spatial Biology
Genomics

Background:

Current epigenomic profiling methods lack spatial resolution.
Understanding genome-wide epigenetic regulation in specific tissue locations is challenging.

Purpose of the Study:

To introduce a novel method for spatially resolved epigenomics.
To enable the discovery of regulatory programs linked to spatial gene expression.

Main Methods:

Integration of transposase-accessible chromatin profiling in tissue sections.
Utilizing barcoded solid-phase capture for spatial epigenomic analysis.

Main Results:

Demonstrated the capability of spatial ATAC for epigenomic profiling with spatial resolution.
Enabled discovery of regulatory programs in mouse organogenesis and lineage differentiation.
Applied to analyze epigenomic alterations in human pathology.

Conclusions:

Spatial ATAC is a powerful tool for spatially resolved epigenomics.
This method advances the understanding of gene regulation in complex biological systems.
Opens new avenues for studying tissue development and disease.