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Updated: Aug 15, 2025
08:07
Three-Dimensional Bone Extracellular Matrix Model for Osteosarcoma
Published on: April 12, 2019
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FGF23 promotes proliferation, migration and invasion by regulating miR-340-5p in osteosarcoma
Lun Fang1, Zhongzhe Li1, Beilei Yu1
1Institute of Sports Medicine, Shandong First Medical University and Shandong Academy Medical Sciences, 619 Changcheng Road, Taian, 271016, Shandong, People's Republic of China.
Journal of Orthopaedic Surgery and Research
|January 5, 2023
Summary
Fibroblast growth factor 23 (FGF23) promotes osteosarcoma progression by targeting microRNA-340-5p (miR-340-5p). This study elucidates FGF23
Area of Science:
- Oncology
- Molecular Biology
- Biochemistry
Background:
- Fibroblast growth factor 23 (FGF23) is implicated in malignant tumor behavior.
- Its specific role and mechanisms in osteosarcoma require further investigation.
Purpose of the Study:
- To investigate the effects of FGF23 on osteosarcoma cell proliferation, migration, and invasion.
- To elucidate the molecular mechanisms underlying FGF23's role in osteosarcoma.
Main Methods:
- Western blot to assess FGF23 expression in osteosarcoma and osteoblast cell lines.
- Cell proliferation, cycle, migration, and invasion assays (MTT, colony formation, flow cytometry, scratch assay, Holomonitor, Transwell).
- Dual-luciferase reporter assay and QRT-PCR to validate FGF23-miR-340-5p interaction and expression levels.
Main Results:
- FGF23 exhibited higher expression in osteosarcoma cells (MG-63, U2-OS) compared to osteoblasts (hFOB1.19).
- FGF23 overexpression significantly enhanced osteosarcoma cell proliferation, migration, and invasion.
- MiR-340-5p was identified as a direct target of FGF23, and its restoration reversed the pro-tumorigenic effects of FGF23.
Conclusions:
- FGF23 promotes osteosarcoma cell proliferation, migration, and invasion.
- This promotion occurs through targeting the gene expression of miR-340-5p.
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