Effects and mechanism of gastrodin supplementation on exercise-induced fatigue in mice

Gastrodin reportedly exerts various pharmacological and health effects. However, the function of gastrodin in reducing exercise-induced fatigue remains elusive. Herein, we investigated the anti-fatigue effects of gastrodin on male mice and explored its possible mechanism of action. At 50 and 100 mg per kg per day, gastrodin significantly reduced fatigue in mice, confirmed using the forced swimming test, whereas no effect was noted at 20 mg per kg per day. Gastrodin preserved muscle and liver glycogen, increased superoxide dismutase activity, and decreased malondialdehyde, blood lactate, and blood urea nitrogen levels. Notably, gastrodin upregulated the mRNA expression levels of AMPK, nuclear factor erythroid 2-related factor 2 (Nrf2), and heme oxygenase 1. Gastrodin also regulated the composition of intestinal flora. These results confirmed that gastrodin activated AMPK and Nrf2/HO-1 pathways and regulated intestinal flora to improve performance in exhaustive exercise, suggesting that gastrodin is an effective functional food to alleviate exercise-induced fatigue.