Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Electron Microscope Tomography and Single-particle Reconstruction

Electron Microscope Tomography and Single-particle Reconstruction

Transmission electron microscopy (TEM) can be used to determine the 3D structure of biological samples with the help of techniques such as electron microscope tomography and single-particle reconstruction. While single-particle reconstruction can examine macromolecules and macromolecular complexes in vitro conditions only, tomography permits the study of cell components or small cells in vivo.
Electron Tomography
Electron tomography can be performed either in TEM or STEM (scanning transmission...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Use of Phase-Angle Model for Full-Field 3D Reconstruction under Efficient Local Calibration.

Sensors (Basel, Switzerland)·2024

Same author

Hot Oscillatory Pressing of Carbon Nanotube-Reinforced Copper Matrix Nanocomposite.

Nanomaterials (Basel, Switzerland)·2021

Same author

Two orders of magnitude extra SERS enhancement on silver nanoparticle-based substrate induced by laser irradiation in nitrogen ambient.

Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy·2021

Same author

Single-step-fabricated disordered metasurfaces for enhanced light extraction from LEDs.

Light, science & applications·2021

Same author

Fatty acids impact sarcomere integrity through myristoylation and ER homeostasis.

Cell reports·2021

Same author

Relationship between obesity and structural brain abnormality: Accumulated evidence from observational studies.

Ageing research reviews·2021

Same journal

Denoising algorithm of Φ-OTDR systems based on adaptive fractional wavelet transform denoising.

Optics express·2026

Same journal

Millisecond photon-to-photon latency and high-speed volumetric projection system for optogenetics.

Optics express·2026

Same journal

Polarization-encoded coaxial structured light for high-precision 3D surface profilometry.

Optics express·2026

Same journal

Discrete freeform optical design based on collaborative optimization of point cloud and local normals.

Optics express·2026

Same journal

Ultrafast ghost imaging with 25 GHz speckle switching and wavelength-division multiplexing.

Optics express·2026

Same journal

Atomic vapor cells fabricated by femtosecond laser welding of standard-optical-quality glass.

Optics express·2026

See all related articles

Search research articles

Related Experiment Video

Updated: Aug 15, 2025

Three-dimensional Imaging of Bacterial Cells for Accurate Cellular Representations and Precise Protein Localization

Three-dimensional Imaging of Bacterial Cells for Accurate Cellular Representations and Precise Protein Localization

Published on: October 29, 2019

Uniaxial MEMS-based 3D reconstruction using pixel refinement.

Min Han, Fengxiao Lei, Weijian Shi

|January 6, 2023

Summary

This summary is machine-generated.

A new fringe projection profilometry (FPP) system uses a micro-electro-mechanical systems (MEMS) mirror for 3D reconstruction. A novel pixel refinement method improves accuracy by excluding outlier pixels during calibration.

More Related Videos

Single Particle Cryo-Electron Microscopy: From Sample to Structure

Single Particle Cryo-Electron Microscopy: From Sample to Structure

Published on: May 29, 2021

Lensfree On-chip Tomographic Microscopy Employing Multi-angle Illumination and Pixel Super-resolution

Lensfree On-chip Tomographic Microscopy Employing Multi-angle Illumination and Pixel Super-resolution

Published on: August 16, 2012

Related Experiment Videos

Last Updated: Aug 15, 2025

Three-dimensional Imaging of Bacterial Cells for Accurate Cellular Representations and Precise Protein Localization

Three-dimensional Imaging of Bacterial Cells for Accurate Cellular Representations and Precise Protein Localization

Published on: October 29, 2019

Single Particle Cryo-Electron Microscopy: From Sample to Structure

Single Particle Cryo-Electron Microscopy: From Sample to Structure

Published on: May 29, 2021

Lensfree On-chip Tomographic Microscopy Employing Multi-angle Illumination and Pixel Super-resolution

Lensfree On-chip Tomographic Microscopy Employing Multi-angle Illumination and Pixel Super-resolution

Published on: August 16, 2012

Area of Science:

Optics and Photonics
Micro-electro-mechanical Systems (MEMS)
3D Metrology

Background:

Fringe Projection Profilometry (FPP) systems are crucial for 3D reconstruction.
Calibration inaccuracies in FPP systems, especially MEMS-based ones, degrade 3D mapping precision.
MEMS projectors with uniaxial vibration mirrors present unique calibration challenges due to lack of focusing optics and unidirectional fringe projection.

Purpose of the Study:

To develop a novel FPP system utilizing a uniaxial MEMS micro-vibration mirror.
To address calibration difficulties inherent in MEMS-based FPP systems.
To enhance the accuracy of 3D reconstruction by mitigating the impact of calibration errors.

Main Methods:

Development of a uniaxial MEMS micro-vibration mirror-based FPP system.
Proposal of a specialized calibration model tailored for MEMS-based FPP systems.
Implementation of a pixel refinement method using a frequency distribution histogram of calibration errors to identify and exclude outlier pixels.

Main Results:

The proposed calibration model and pixel refinement method effectively address calibration issues in the MEMS-based FPP system.
Outlier pixels with significant calibration errors were successfully identified and excluded from the 3D mapping process.
Experimental validation confirmed a notable improvement in the accuracy of 3D reconstruction.

Conclusions:

The developed FPP system with a uniaxial MEMS micro-vibration mirror is feasible for 3D metrology.
The proposed calibration and pixel refinement techniques significantly enhance the precision of 3D reconstruction in MEMS-based FPP systems.
This work offers a viable solution for improving the accuracy of 3D measurements using MEMS technology.