Different view on diffraction-limited imaging optics design
Related Experiment Video
Updated: Aug 15, 2025

High-Throughput Total Internal Reflection Fluorescence and Direct Stochastic Optical Reconstruction Microscopy Using a Photonic Chip
Published on: November 16, 2019
Abstract:
The task of imaging optics design starts with choosing a subset of imaging parameters such as the object distance, the image magnification, the image resolution, and the depth of field. It is clear that the imaging parameters are inter-related and their appropriate trade-off is the key problem of final optical design. We provide a novel analysis of the optical design considering diffractive imaging phenomena and thin lens approximation, and we derive formulas for the solution of individual parameters. We show that combinations of three independent optical design input variables determine all other optical and geometrical parameters under diffraction-limited optical imaging. We also formulate an invariant relation in the object space if the depth of field is one of the design's target parameters.
More Related Videos
Related Concept Videos
Imaging Biological Samples with Optical Microscopy
In optical microscopy, the specimen to be viewed is placed on a glass slide and clipped on the stage...
Focusing of Light in the Eye
Confocal Fluorescence Microscopy
X-ray Crystallography
Diffraction
Diffraction is the change in the direction of travel experienced by an electromagnetic wave when it encounters a physical barrier whose dimensions are comparable to those of the wavelength of the light. X-rays are electromagnetic radiation with wavelengths about as long as the distance between neighboring...
Phase Contrast and Differential Interference Contrast Microscopy
In-phase-contrast microscopes, interference between light directly passing through a cell and light refracted by cellular components is used to create high-contrast, high-resolution images without staining. It is the oldest and simplest type of microscope that creates an image by altering the wavelengths of light rays passing through the specimen. Altered wavelength paths are created using an annular stop in the condenser. The annular stop produces a hollow cone of...
Super-resolution Fluorescence Microscopy

