Comparison of three different methods used in the preparation of leukocyte-poor platelet concentrates

We compared three methods for preparing leukocyte-poor platelet concentrates from random donors: the centrifugation procedure 400 g/6 min, the filtration by Immugard TFIG 500 Y and the Cutter Leukotrap platelet pooling system. The most efficient method with regard to removal of leukocytes and platelet yield could be obtained by filtration through the Immugard filter. Platelet loss was less than 10% and the residual leukocyte count was lower than 10 X 10(6).