Protocol for in vivo lineage tracing of the mouse-papillomavirus-type 1-infected cells in mice
Vural Yilmaz1, Katerina Strati1
1Department of Biological Sciences, University of Cyprus, Nicosia 2109, Cyprus.
Abstract:
Here, we present a protocol to create an in vivo lineage-tracing mouse model for mouse-papillomavirus-type 1 (MmuPV1)-infected cells. We describe the steps to generate and deliver the MmuPV1 lox-Cre-lox plasmid for the infection of mice, followed by skin tissue extraction and processing. We then detail how to use flow cytometry to trace, quantify, and analyze MmuPV1-harboring cells and their progeny. This model is suitable to investigate the early effects of papillomavirus on the target cells. For complete details on the use and execution of this protocol, please refer to Yilmaz et al. (2022).1.
Insights
This study introduces a new mouse model to track mouse papillomavirus type 1 (MmuPV1) infections. The model allows researchers to study how MmuPV1 affects cells early in infection.
Area of Science:
- Virology
- Immunology
- Molecular Biology
Background:
- Mouse papillomavirus type 1 (MmuPV1) is a relevant model for studying papillomavirus infections.
- Understanding the early cellular effects of MmuPV1 is crucial for developing effective treatments.
Purpose of the Study:
- To present a protocol for an in vivo lineage-tracing mouse model for MmuPV1-infected cells.
- To enable the investigation of early papillomavirus effects on target cells.
Main Methods:
- Generation and delivery of a MmuPV1 lox-Cre-lox plasmid for mouse infection.
- Skin tissue extraction and processing.
- Flow cytometry for tracing, quantifying, and analyzing MmuPV1-harboring cells and their progeny.
Main Results:
- A functional lineage-tracing mouse model for MmuPV1 infection was successfully established.
- The protocol allows for detailed analysis of MmuPV1-infected cell populations and their descendants.
Conclusions:
- The developed mouse model provides a valuable tool for studying the initial stages of papillomavirus infection.
- This model facilitates research into the cellular dynamics and early pathogenesis of MmuPV1.
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