Rapid simultaneous analysis of anti human immunodeficiency virus drugs in pharmaceutical formulation by smart...

In this study, two chemometrics methods, including partial least squares regression (PLS) and least squares support vector machine (LS-SVM) were applied for the simultaneous determination of zidovudine (ZDV) and lamivudine (LMV) in synthetic mixtures and anti-HIV pharmaceutical formulation. These approaches along with the spectrophotometric method were used to solve spectral overlapping problems between mentioned components. The results of PLS showed that the number of components for ZDV and LMV were 10 and 10 with mean square prediction error (MSPE) of 0.4045 and 2.1189, respectively. This method revealed recoveries ranging from 99.48% to 100.40% and 99.55% to 101.25% for ZDV and LMV, respectively. By applying leave-one-out cross-validation (LOO-CV), γ (regularization parameter) and σ² (width of the function) values were found to be 50, 1500 and 210, 20 with root mean square error (RMSE) of 0.6156 and 0.3163 for ZDV and LMV, respectively. The mean recoveries obtained by the LS-SVM were 100.82% and 98.93% for ZDV and LMV, respectively. A comparison between the suggested methods and high-performance liquid chromatography (HPLC) as a reference technique was implemented, which did not show a significant difference. The results obtained in this research revealed that the chemometrics approaches can be efficient, simple, inexpensive, and precise for routine analysis and quality control of the drug.