Smartphone-based on-site detection of hydrogen peroxide in milk by using a portable ratiometric fluorescent probe

The on-site detection of hydrogen peroxide (H₂O₂) is important for maintaining food safety as the ingestion of H₂O₂ can lead to serious pathological conditions. However, most reported fluorescent probes require a fluorometer to ensure readable signal output and reliable detection result. Consequently, the fluorescent detection of H₂O₂ can be realized only within a standard laboratory setting. Herein, we report a novel supramolecular strategy that can successfully convert the typical off-on response to H₂O₂ into a ratiometric response, which allows the on-site detection of H₂O₂ when used in conjunction with a smartphone-based 3D-printed miniaturized testing system. This method has acceptable sensitivity, good anti-interference ability, and desirable accuracy compared to a standard detection method. More importantly, this portable ratiometric method can be used to detect H₂O₂ residue in commercial milk samples with the simple testing apparatuses.