Formulation and development of novel lipid-based combinatorial advanced nanoformulation for effective treatment of...

Area of Science:

• Dermatology
• Nanotechnology
• Oncology

Background:

• Non-melanoma skin cancer presents significant morbidity and challenges due to drug resistance.
• Conventional therapies often lack sufficient chemotherapeutic efficacy for skin cancers.
• Nanoparticles offer a promising approach for targeted drug delivery and enhanced cancer cell interaction.

Purpose of the Study:

• To develop and evaluate a novel nanoparticle-based combinatorial chemotherapy for non-melanoma skin cancer.
• To investigate the efficacy of 5-fluorouracil (5-FU) and cannabidiol (CBD) loaded nanostructured lipid carrier gel (FU-CBD-NLCs gel).

Main Methods:

• Nanostructured lipid carriers (NLCs) loaded with 5-FU and CBD were optimized using a central composite design.
• In vitro and ex vivo drug permeation studies were conducted to assess skin delivery.
• In vitro cell studies (MTT assays, wound healing, cell cycle analysis) and dermatokinetic studies were performed.

Main Results:

• Optimized NLCs exhibited an average particle size of 206 nm and zeta potential of -34 mV.
• Effective delivery of both 5-FU and CBD into skin layers was demonstrated via nanocarriers.
• FU-CBD-NLCs showed superior in vitro efficacy and enhanced drug deposition in epidermal and dermal layers compared to conventional formulations.

Conclusions:

• The developed FU-CBD-NLCs gel represents a novel combinatorial chemotherapy approach.
• This nanoparticle formulation offers a promising therapeutic option for non-melanoma skin cancer treatment.
• The study highlights the potential of nanocarriers to overcome drug resistance and improve treatment outcomes.