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A 'print-pause-print' protocol for 3D printing microfluidics using multimaterial stereolithography.

Yong Tae Kim1, Alireza Ahmadianyazdi2, Albert Folch2

  • 1Department of Chemical Engineering & Biotechnology, Tech University of Korea, Siheung-si, Republic of Korea. ytkim@tukorea.ac.kr.

Nature Protocols
|January 7, 2023
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Summary
This summary is machine-generated.

3D printing enables rapid fabrication of transparent, biocompatible microfluidic chips. This new method allows for multimaterial devices with distinct properties, advancing applications in cell biology and tissue engineering.

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Area of Science:

  • Biomedical Engineering
  • Materials Science
  • Microfluidics

Background:

  • 3D printing offers rapid, low-intervention fabrication of microfluidic devices from digital designs.
  • Current challenges include creating high-resolution chips that are transparent, biocompatible, and multimaterial.

Purpose of the Study:

  • To detail strategies for fabricating transparent biomicrofluidic devices and multimaterial chips using stereolithographic 3D printing.
  • To demonstrate the utility of these chips in biological applications and material diffusion studies.

Main Methods:

  • Developed a transparent resin using poly(ethylene glycol) diacrylate (PEG-DA-250) for chip fabrication.
  • Employed a 'print-pause-print' protocol with stereolithography to enable multimaterial printing.
  • Utilized a glass surface technique for achieving smooth chip surfaces.

Main Results:

  • Successfully fabricated transparent microfluidic chips using PEG-DA-250, suitable for cell culture and visualization.
  • Demonstrated multimaterial fabrication with water-impermeable channel walls and porous, permeable barriers using different PEG-DA resins.
  • Achieved automatic alignment of dissimilar materials within an hour-long printing process.

Conclusions:

  • The developed 'print-pause-print' stereolithographic 3D printing protocol facilitates the creation of advanced transparent and multimaterial microfluidic devices.
  • This technique supports diverse chip-based applications, including biomolecule analysis, cell biology, organ-on-a-chip systems, and tissue engineering.