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Lysophospholipase activity of Mycobacterium smegmatis
1Department of Microbiology, Southeastern College of Osteopathic Medicine, North Miami Beach, Florida 33162-3097.
Zentralblatt Fur Bakteriologie, Mikrobiologie, Und Hygiene. Series A, Medical Microbiology, Infectious Diseases, Virology, Parasitology
|May 1, 1987
Abstract:
Lysophospholipase (LPL) activity found in Mycobacterium smegmatis was characterized. LPL activity was found to exist in high levels in two-to-10-day-old cultures. The enzyme assay was run using 1.4 X 10(9) viable bacteria at an incubation time of 24 h. Enzyme activity was detected between 1 degree C and 80 degrees C with maximal activity between 40 degrees C and 60 degrees C. LPL activity was maximal at pH 9.0, with activity ranging between pH 3.0 and 10.0.