Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Phase Contrast and Differential Interference Contrast Microscopy01:26

Phase Contrast and Differential Interference Contrast Microscopy

8.3K
Phase-Contrast Microscopes
In-phase-contrast microscopes, interference between light directly passing through a cell and light refracted by cellular components is used to create high-contrast, high-resolution images without staining. It is the oldest and simplest type of microscope that creates an image by altering the wavelengths of light rays passing through the specimen. Altered wavelength paths are created using an annular stop in the condenser. The annular stop produces a hollow cone of...
8.3K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Inhibition of lysine-specific demethylase 1 (LSD1) prevented tumor growth and metastasis by downregulating PD-L1 expression in lung adenocarcinoma.

Genes & diseases·2023
Same author

Thermally Reversible Cross-Linking of Recyclable Polyamide Materials Based on Schiff Base and Diels-Alder Reactions.

Macromolecular rapid communications·2023
Same author

An Fgr kinase inhibitor attenuates sepsis-associated encephalopathy by ameliorating mitochondrial dysfunction, oxidative stress, and neuroinflammation via the SIRT1/PGC-1α signaling pathway.

Journal of translational medicine·2023
Same author

Photocurable 3D-printed PMBG/TCP biphasic scaffold mimicking vasculature for bone regeneration.

International journal of bioprinting·2023
Same author

Combined Transcriptome and Proteome Analysis Provides Insights into Petaloidy in Pomegranate.

Plants (Basel, Switzerland)·2023
Same author

RNF216 affects the stability of STAU2 in the hypothalamus.

Development, growth & differentiation·2023

Related Experiment Video

Updated: Aug 15, 2025

Quantitative Optical Microscopy: Measurement of Cellular Biophysical Features with a Standard Optical Microscope
14:09

Quantitative Optical Microscopy: Measurement of Cellular Biophysical Features with a Standard Optical Microscope

Published on: April 7, 2014

15.7K

Retinex-qDPC: Automatic background-rectified quantitative differential phase contrast imaging.

Shuhe Zhang1, Tao Peng2, Zeyu Ke2

  • 1School of Biomedical Engineering, Anhui Medical University, Hefei 230032, China; University Eye Clinic Maastricht, Maastricht University Medical Center +, P.O. Box 5800, Maastricht, AZ 6202, the Netherlands.

Computer Methods and Programs in Biomedicine
|January 7, 2023
PubMed
Summary
This summary is machine-generated.

Quantitative differential phase contrast reconstruction (qDPC) quality is improved by Retinex-qDPC, a novel method enhancing experimental robustness against background mismatches. This approach offers superior phase recovery without optical system modifications.

Keywords:
Background correctionDeconvolutionDifferential phase contrast imagingQuantitative phase retrievalRetinex theory

More Related Videos

Phase Contrast and Differential Interference Contrast DIC Microscopy
06:49

Phase Contrast and Differential Interference Contrast DIC Microscopy

Published on: August 6, 2008

52.5K
A TIRF Microscopy Technique for Real-time, Simultaneous Imaging of the TCR and its Associated Signaling Proteins
16:10

A TIRF Microscopy Technique for Real-time, Simultaneous Imaging of the TCR and its Associated Signaling Proteins

Published on: March 22, 2012

24.0K

Related Experiment Videos

Last Updated: Aug 15, 2025

Quantitative Optical Microscopy: Measurement of Cellular Biophysical Features with a Standard Optical Microscope
14:09

Quantitative Optical Microscopy: Measurement of Cellular Biophysical Features with a Standard Optical Microscope

Published on: April 7, 2014

15.7K
Phase Contrast and Differential Interference Contrast DIC Microscopy
06:49

Phase Contrast and Differential Interference Contrast DIC Microscopy

Published on: August 6, 2008

52.5K
A TIRF Microscopy Technique for Real-time, Simultaneous Imaging of the TCR and its Associated Signaling Proteins
16:10

A TIRF Microscopy Technique for Real-time, Simultaneous Imaging of the TCR and its Associated Signaling Proteins

Published on: March 22, 2012

24.0K

Area of Science:

  • Microscopy and Imaging
  • Computational Imaging
  • Phase Contrast Microscopy

Background:

  • Quantitative differential phase contrast reconstruction (qDPC) quality is degraded by background mismatches in oblique illuminated images.
  • Manual background calibration is time-consuming, unstable, and impossible for dynamic or defocusing layers.
  • Background mismatches limit qDPC's experimental robustness and applications.

Purpose of the Study:

  • To develop a robust qDPC method that addresses background mismatches.
  • To enhance the experimental robustness and applicability of qDPC.

Main Methods:

  • Proposed Retinex-qDPC, replacing the data fidelity term with image edge features.
  • Developed L2-Retinex-qDPC and L1-Retinex-qDPC models for robust reconstruction.
  • Employed the split Bregman method for L1-Retinex-qDPC and compared against state-of-the-art algorithms.

Main Results:

  • Retinex-qDPC significantly improves phase recovery by suppressing background mismatch impact.
  • L1-Retinex-qDPC demonstrated superior performance compared to L2-Retinex and other qDPC algorithms.
  • Both simulated and experimental data validated the effectiveness of the proposed methods.

Conclusions:

  • Retinex-qDPC enhances experimental robustness against background variations.
  • The method requires no optical system modification, benefiting all qDPC applications.
  • Retinex-qDPC offers a stable and versatile solution for improved qDPC reconstruction.