Related Experiment Video
Updated: Aug 15, 2025
06:53
Rapid Antibody Glycoengineering in Chinese Hamster Ovary Cells
Published on: June 2, 2022
3.3K
CHOGlycoNET: Comprehensive glycosylation reaction network for CHO cells
Pavlos Kotidis1, Roberto Donini2, Johnny Arnsdorf3
1Sargent Centre for Process Systems Engineering, Department of Chemical Engineering, Imperial College London, London, SW7 2AZ, UK.
Metabolic Engineering
|January 7, 2023
Summary
Chinese hamster ovary (CHO) cells are crucial for glycoprotein therapeutics. This study introduces CHOGlycoNET, a comprehensive reaction network model, to better understand and predict protein glycosylation for improved biomanufacturing.
Area of Science:
- Biotechnology
- Glycobiology
- Systems Biology
Background:
- Chinese hamster ovary (CHO) cells are vital for producing glycoprotein therapeutics.
- N-linked glycans are critical quality attributes influencing therapeutic protein activity and immunogenicity.
- Current glycosylation models are often product-specific, limiting broader application.
Purpose of the Study:
- To reconstruct a comprehensive glycosylation reaction network for CHO cells.
- To investigate enzyme distribution and identify key reactions in protein glycosylation.
- To facilitate the development of predictive glycomodels and glycoengineering strategies.
Main Methods:
- Compilation of 200 glycan datasets from CHO cell lines.
- Reconstruction of a comprehensive reaction network (CHOGlycoNET) from minimal reaction networks.
- Application of machine learning and dimensionality reduction techniques.
Main Results:
- Development of CHOGlycoNET, a unified glycosylation reaction network for CHO cells.
- Identification of key enzymatic reactions and Golgi apparatus enzyme distribution.
- Demonstration of CHOGlycoNET's utility in predicting glycoengineering outcomes.
Conclusions:
- CHOGlycoNET provides a robust framework for understanding CHO cell glycosylation.
- The model accelerates the development of glycomodels and aids in designing glycoengineering strategies.
- CHOGlycoNET is available as an SBML file for integration with other biological models.
Related Concept Videos
01:25Protein Glycosylation
7.1K
Glycosylation, the most common post-translational modification for proteins, serves diverse functions. Adding sugars to proteins makes the proteins more resistant to proteolytic digestion. Glycosylated proteins can act as markers and receptors to promote cell-cell adhesion. Additionally, they have many essential quality control functions in the cell, such as correct protein folding and facilitating transport of misfolded proteins to the cytosol, which can be degraded.
Glycosylation occurs in...
Glycosylation occurs in...
7.1K
01:24Oligosaccharide Assembly
2.9K
Protein glycosylation starts in the ER lumen and continues in the Golgi apparatus. Glycosyltransferases catalyze the addition of sugar molecules or glycosylation of proteins. Usually, these enzymes add sugars to the hydroxyl groups of selected serine or threonine residues to form O-linked glycans or the amino groups of asparagine residues to form N-linked glycans. Different positions on the same polypeptide chain can contain differently linked glycans.
Multiple sugar molecules that may or may...
Multiple sugar molecules that may or may...
2.9K
01:36Carbohydrate Metabolism
11.4K
Carbohydrates are polymers composed of molecules containing atoms of carbon, hydrogen and oxygen. One gram of carbohydrate can provide four kilo-calories of energy, which makes it the most efficient instant energy source.
Starch accounts for approximately 60% of the carbohydrates consumed by humans. Since amylase enzymes cannot function in the stomach's acidic environment, starch can only be digested in the mouth and small intestine. Simple sugars are found naturally in milk and fruits in...
Starch accounts for approximately 60% of the carbohydrates consumed by humans. Since amylase enzymes cannot function in the stomach's acidic environment, starch can only be digested in the mouth and small intestine. Simple sugars are found naturally in milk and fruits in...
11.4K
01:26Biosynthesis of Polysaccharides
52
Polysaccharides such as glycogen and starch are synthesized from nucleoside diphosphate sugars, primarily uridine diphosphate glucose (UDPG) and adenosine diphosphate glucose (ADPG). These activated glucose donors act as key intermediates in carbohydrate metabolism and biosynthesis. UDPG primarily involves glycogen synthesis in animals and many bacteria, while ADPG plays a fundamental role in starch synthesis in plants and certain bacteria.UDPG is formed when glucose-1-phosphate reacts with...
52
01:19Overview of Carbohydrate Metabolism
1.4K
Carbohydrate metabolism is a fundamental biochemical process that ensures a constant supply of energy to living cells. The most important carbohydrate is glucose, which can be broken down via glycolysis to enter into the Krebs cycle and eventually lead to the production of ATP through oxidative phosphorylation.
Glucose transport into cells is facilitated by a family of transport proteins called GLUT (Glucose Transporters). GLUT4 is the primary glucose transporter for insulin-stimulated glucose...
Glucose transport into cells is facilitated by a family of transport proteins called GLUT (Glucose Transporters). GLUT4 is the primary glucose transporter for insulin-stimulated glucose...
1.4K
01:27Glucose Transporters
23.9K
Glucose transporters facilitate the transport of glucose across the cell membrane. In addition to glucose, some glucose transporters can also aid the movement of other hexoses such as fructose, mannose, and galactose.
Facilitated diffusion-glucose transporters (GLUTs) are encoded by the solute-linked carrier (SLC) family 2, subfamily A gene family, or SLC2A. The 14 GLUT protein members are distributed into three classes:
Facilitated diffusion-glucose transporters (GLUTs) are encoded by the solute-linked carrier (SLC) family 2, subfamily A gene family, or SLC2A. The 14 GLUT protein members are distributed into three classes:
23.9K