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LncRNA-mRNA expression profile and functional network of vascular dysfunction in septic rats
Ye-Chen Han1, Zhu-Jun Shen1, Yi-Ning Wang1
1Department of Cardiology, Peking Union Medical College Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, No.1 Shuaifuyuan, Dongcheng District, Beijing, 100730, China.
European Journal of Medical Research
|January 7, 2023
Summary
This study analyzed long non-coding RNAs (lncRNAs) and mRNAs in rat aorta tissue during sepsis. Key findings reveal altered lncRNA and mRNA expression, impacting mitochondrial function and energy metabolism, offering potential therapeutic targets for sepsis.
Area of Science:
- Molecular Biology
- Genomics
- Biochemistry
Background:
- Sepsis significantly alters gene expression in aorta tissue.
- Long non-coding RNAs (lncRNAs) and messenger RNAs (mRNAs) play crucial roles in sepsis pathogenesis.
- Understanding lncRNA-mRNA interactions is vital for elucidating sepsis mechanisms.
Purpose of the Study:
- To analyze differential expression of lncRNAs and mRNAs in a rat model of sepsis.
- To construct functional networks (lncRNA-mRNA, lncRNA-miRNA-mRNA) in sepsis.
- To identify potential molecular mechanisms and therapeutic targets for sepsis.
Main Methods:
- Microarray analysis of lncRNA and mRNA expression profiles in rat aorta tissue.
- Intraperitoneal lipopolysaccharide injection to induce sepsis in Wistar rats.
- Bioinformatics analyses including Gene Ontology, Kyoto Encyclopedia of Genes and Genomes pathway enrichment, coding/non-coding co-expression, and competing endogenous RNA (ceRNA) networks.
Main Results:
- Identified 503 differentially expressed lncRNAs and 2479 differentially expressed mRNAs in septic rats.
- Down-regulated mRNAs were associated with mitochondrial structure, function, and energy metabolism pathways.
- Validated lncRNAs regulate energy metabolism and mitochondrial dynamics; ceRNA analysis suggests roles in inflammatory response.
Conclusions:
- Microarray analysis successfully identified differentially expressed lncRNAs and mRNAs in the aorta of septic rats.
- These findings highlight the role of lncRNAs in sepsis pathogenesis at the genetic level.
- Further investigation of these lncRNAs may uncover novel therapeutic strategies for sepsis.
