Related Experiment Video

Updated: Jun 16, 2026

High-throughput Detection of Respiratory Pathogens in Animal Specimens by Nanoscale PCR
11:00

High-throughput Detection of Respiratory Pathogens in Animal Specimens by Nanoscale PCR

Published on: November 28, 2016

12.0K

Molecular Epidemiology of Pasteurella multocida Associated with Bovine Respiratory Disease Outbreaks

Johan Manuel Calderón Bernal1, Ana Fernández2, José Luis Arnal2

  • 1Departamento de Sanidad Animal, Facultad de Veterinaria, Universidad Complutense, 28040 Madrid, Spain.

Animals : an Open Access Journal From MDPI
|January 8, 2023
PubMed

Insights

Bovine respiratory disease (BRD)-associated Pasteurella multocida isolates show low genetic diversity. Most isolates belong to a single genotype (A:L3), indicating a clonal population structure in cattle.

Area of Science:

  • Veterinary Microbiology
  • Bacterial Pathogenesis
  • Animal Health

Background:

  • Bovine respiratory disease (BRD) is a significant economic concern in cattle production.
  • Pasteurella multocida is a key bacterial pathogen implicated in BRD outbreaks.
  • Limited characterization data exists for BRD-associated P. multocida isolates compared to other hosts.

Purpose of the Study:

  • To genetically characterize P. multocida isolates from BRD outbreaks.
  • To determine the population structure and genetic diversity of these isolates.
  • To identify prevalent capsular types, LPS genotypes, and virulence factors.

Main Methods:

  • Characterization of 170 P. multocida isolates from 125 BRD outbreaks.
  • Capsular typing, LPS typing, and virulotyping.
  • Multilocus sequence typing (MLST) and pulsed-field gel electrophoresis (PFGE) for genetic relatedness analysis.

Main Results:

  • Low genetic diversity observed across all typing methods (GD=0.02 for capsular/LPS, GD=0.18 for PFGE).
  • Predominance of capsular type A and LPS genotype L3, with 97.6% of isolates as A:L3.
  • MLST identified ST79 and ST13 as most frequent, grouped within clonal complex CC13.
  • PFGE revealed a single pattern in 62.5% of outbreaks with multiple isolates analyzed.

Conclusions:

  • BRD-associated P. multocida exhibits a clonal population structure.
  • The findings suggest a limited number of P. multocida clones are responsible for BRD in cattle.
  • This genetic relatedness has implications for understanding disease transmission and control strategies.
Keywords:
MLSTPFGEPasteurella multocidabovine respiratory diseasecattleoutbreaksvirulotyping

More Related Videos

The Bovine Lung in Biomedical Research: Visually Guided Bronchoscopy, Intrabronchial Inoculation and In Vivo Sampling Techniques09:01

The Bovine Lung in Biomedical Research: Visually Guided Bronchoscopy, Intrabronchial Inoculation and In Vivo Sampling Techniques

Published on: July 3, 2014

23.3K
Cost-effective Method for Microbial Source Tracking Using Specific Human and Animal Viruses11:29

Cost-effective Method for Microbial Source Tracking Using Specific Human and Animal Viruses

Published on: December 3, 2011

12.3K

Related Concept Videos

Inhalation Anthrax01:25

Inhalation Anthrax

Anthrax is a zoonotic disease caused by Bacillus anthracis, a Gram-positive, spore-forming bacterium. It primarily affects herbivorous animals but can be transmitted to humans through skin contact, ingestion, or inhalation of spores.Cutaneous anthrax, the most common form, typically results from direct contact with bacterial spores through skin abrasions and is generally less severe. Gastrointestinal anthrax results from eating undercooked or contaminated meat. It affects the mouth, throat, or...
Clinical Significance of Antibiotic Resistance01:25

Clinical Significance of Antibiotic Resistance

Methicillin-resistant Staphylococcus aureus (MRSA) presents a critical public health threat, arising from its capacity to resist β-lactam antibiotics due to acquisition of the mecA gene within the staphylococcal cassette chromosome mec (SCCmec). This gene encodes penicillin-binding protein 2a (PBP2a), which impairs binding efficacy of methicillin and other β-lactams. MRSA has evolved into distinct clonal lineages impacting humans and animals alike, reinforcing its significance within the One...
Atypical Pneumonia01:14

Atypical Pneumonia

Atypical pneumonia, often caused by Mycoplasma pneumoniae, is a form of pulmonary infection that differs from the classical presentation of bacterial pneumonia in both its cause and clinical symptoms. Mycoplasma pneumoniae is a pleomorphic bacterium notable for its lack of a rigid cell wall. This structural characteristic imparts resistance to beta-lactam antibiotics and significantly influences the bacterium’s behavior within the human host.Other pathogens responsible for the disease include...
Investigation of Disease Outbreaks01:23

Investigation of Disease Outbreaks

Multistate foodborne outbreaks pose significant public health risks and require meticulous investigation to identify sources and implement control measures. The Centers for Disease Control and Prevention (CDC) utilizes a dynamic seven-step process for these investigations, integrating data from laboratories, interviews, and environmental assessments to protect public health.Outbreak Detection: The detection of multistate outbreaks typically begins with PulseNet, the CDC's national laboratory...