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CRISPR/Cas-Based Approaches to Study Schizophrenia and Other Neurodevelopmental Disorders
Artemiy O Kurishev1, Dmitry S Karpov1,2, Nonna I Nadolinskaia3
1Mental Health Research Center, Kashirskoe sh. 34, 115522 Moscow, Russia.
International Journal of Molecular Sciences
|January 8, 2023
Summary
CRISPR/Cas systems offer advanced tools for studying complex central nervous system (CNS) diseases. These high-throughput genetic technologies enable detailed investigation of genotype-phenotype relationships in neuronal cells.
Area of Science:
- Neuroscience
- Genetics
- Molecular Biology
Background:
- Studying central nervous system (CNS) diseases at the molecular level is complex due to intricate neural circuits and diverse cell types.
- Genomic studies highlight the complex genetic basis of mental disorders like schizophrenia.
- Investigating these genetic complexities necessitates high-throughput cellular models.
Purpose of the Study:
- To review innovative CRISPR-based approaches for studying molecular mechanisms of CNS pathologies.
- To highlight the utility of CRISPR/Cas systems in creating advanced genetic tools for neuroscience research.
- To discuss the application of these tools in cellular models for understanding genotype-phenotype relationships.
Main Methods:
- Utilizing CRISPR/Cas systems for high-throughput genetic manipulation in cellular models.
- Employing CRISPR/Cas tools to alter DNA sequences and gene functions.
- Investigating genotype-phenotype correlations in neuronal cells.
Main Results:
- CRISPR/Cas systems provide the necessary complexity, versatility, and flexibility for genetic manipulation.
- These systems enable the creation of novel genetic tools for studying gene function.
- CRISPR/Cas tools facilitate the investigation of intricate genotype-phenotype relationships in CNS disorders.
Conclusions:
- CRISPR-based technologies are crucial for advancing the molecular understanding of CNS pathologies.
- Cellular models combined with CRISPR/Cas systems offer powerful approaches to decipher complex genetic architectures.
- This review underscores the potential of CRISPR/Cas systems in driving future discoveries in neurodegenerative and mental health research.
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