Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

A novel biomimetic opto-electronic nose combining cross-reactive peptides with selective phage display peptides for enhanced performance.

Biosensors & bioelectronics·2026
Same author

Unveiling the Interactions between a Protein Derived from Barnacle Adhesive and Surfaces Using Surface Plasmon Resonance Imaging.

ACS applied bio materials·2026
Same author

Biosynthesis of Silver Nanoparticles from <i>Paullinia cupana</i> Kunth Leaf: Effect of Seasonality and Preparation Method of Aqueous Extracts.

Pharmaceuticals (Basel, Switzerland)·2026
Same author

Development of a Methylene Blue Carrier System in Gellan Gum for Application in Antimicrobial Photodynamic Therapy against <i>Candida albicans</i>.

ACS omega·2025
Same author

Effects of the Addition of Aminosilane-Functionalized Titanate Nanotubes in Carboxymethylcellulose-Based Film for Biomedical Applications.

Journal of biomedical materials research. Part B, Applied biomaterials·2025
Same author

Peptides derived from the POU domain of BRN2 show antitumor activity against murine melanoma model cells in vitro and in vivo.

Cancer chemotherapy and pharmacology·2025

Related Experiment Video

Updated: Aug 15, 2025

Real Time Measurements of Membrane Protein:Receptor Interactions Using Surface Plasmon Resonance SPR
09:35

Real Time Measurements of Membrane Protein:Receptor Interactions Using Surface Plasmon Resonance SPR

Published on: November 29, 2014

22.9K

Interaction between Nanoparticles, Membranes and Proteins: A Surface Plasmon Resonance Study.

Erenildo Ferreira de Macedo1,2, Nivia Salles Santos1,2, Lucca Silva Nascimento1

  • 1Laboratory of Nanomaterials and Nanotoxicology, Instituto de Ciência e Tecnologia, Universidade Federal de São Paulo (UNIFESP), São José dos Campos 12231280, SP, Brazil.

International Journal of Molecular Sciences
|January 8, 2023
PubMed
Summary

This study developed a surface plasmon resonance imaging method to analyze nanoparticle interactions with cell membranes and proteins. Gold nanoparticles preferentially bind to fibrinogen, while silver nanoparticles show higher interaction with immunoglobulin G.

Keywords:
affinitybiomolecular interactionsblood serum proteinsmembranesnanocomplex dissociation ratenanoparticlesprotein coronasurface plasmon resonance imaging

More Related Videos

Author Spotlight: Single-Molecule Surface-Enhanced Raman Scattering Measurements Enabled by Plasmonic DNA Origami Nanoantennas
10:43

Author Spotlight: Single-Molecule Surface-Enhanced Raman Scattering Measurements Enabled by Plasmonic DNA Origami Nanoantennas

Published on: July 21, 2023

3.4K
Measurement of Scattering Nonlinearities from a Single Plasmonic Nanoparticle
15:06

Measurement of Scattering Nonlinearities from a Single Plasmonic Nanoparticle

Published on: January 3, 2016

12.9K

Related Experiment Videos

Last Updated: Aug 15, 2025

Real Time Measurements of Membrane Protein:Receptor Interactions Using Surface Plasmon Resonance SPR
09:35

Real Time Measurements of Membrane Protein:Receptor Interactions Using Surface Plasmon Resonance SPR

Published on: November 29, 2014

22.9K
Author Spotlight: Single-Molecule Surface-Enhanced Raman Scattering Measurements Enabled by Plasmonic DNA Origami Nanoantennas
10:43

Author Spotlight: Single-Molecule Surface-Enhanced Raman Scattering Measurements Enabled by Plasmonic DNA Origami Nanoantennas

Published on: July 21, 2023

3.4K
Measurement of Scattering Nonlinearities from a Single Plasmonic Nanoparticle
15:06

Measurement of Scattering Nonlinearities from a Single Plasmonic Nanoparticle

Published on: January 3, 2016

12.9K

Area of Science:

  • Biomedical Engineering
  • Nanotechnology
  • Surface Science

Background:

  • Nanoparticles (NPs) show promise in biomedicine, but their toxic effects raise safety concerns.
  • Direct NP contact with biomolecules or cell membranes can cause cytotoxicity.
  • Understanding NP-biomolecule interactions is crucial for safe nanomaterial development.

Purpose of the Study:

  • To develop a fast and accurate method for characterizing nanoparticle interactions with proteins and lipidic membranes.
  • To investigate the adsorption of gold nanoparticles onto different lipidic membrane compositions.
  • To analyze the binding kinetics of gold and silver nanoparticles with blood proteins.

Main Methods:

  • Utilized Surface Plasmon Resonance Imaging (SPRi) technique.
  • Evaluated gold NP interaction with mimetic lipidic membranes (POPC/cholesterol, POPC, POPC/DDAB).
  • Analyzed gold and silver NP interactions with blood proteins (fibrinogen, immunoglobulin G, etc.) by measuring association/dissociation kinetics.

Main Results:

  • Surface concentration density of gold NPs on POPC/cholesterol membranes was 2.5 times higher than on POPC or POPC/DDAB membranes.
  • Gold NPs exhibited preferential binding to fibrinogen, with 8 times higher reflectivity variation compared to other proteins.
  • Silver NPs showed a 2-fold higher reflectivity variation on immunoglobulin G (IgG) compared to other tested proteins.

Conclusions:

  • SPRi is an effective technique for characterizing NP-biomolecule and NP-membrane interactions.
  • Lipidic membrane composition significantly influences gold NP adsorption.
  • Gold and silver nanoparticles display distinct binding preferences for specific blood proteins, relevant for understanding their biological fate and potential toxicity.