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Updated: Aug 15, 2025

A Method for Measuring RNA N6-methyladenosine Modifications in Cells and Tissues
08:56

A Method for Measuring RNA N6-methyladenosine Modifications in Cells and Tissues

Published on: December 5, 2016

11.0K

Emerging Mutual Regulatory Roles between m6A Modification and microRNAs

Zongqin Mei1, Yahao Mou1, Nan Zhang1

  • 1Institute of Preventive Medicine, School of Public Health, Dali University, No. 22 Wanhua Road, Dali 671000, China.

International Journal of Molecular Sciences
|January 8, 2023
PubMed

Insights

N6-methyladenosine (m6A) is a key RNA modification regulating gene expression. This review details how m6A and its associated proteins impact microRNA production and function in disease.

Area of Science:

  • Epigenetics
  • Molecular Biology
  • RNA Biology

Background:

  • N6-methyladenosine (m6A) is a prevalent RNA modification in mammals, influencing numerous physiological and pathological processes.
  • m6A modification is reversible and dynamically regulated by methyltransferases, demethylases, and methyl-binding proteins.
  • Emerging evidence highlights a significant link between m6A modification and the biogenesis and function of microRNAs (miRNAs).

Purpose of the Study:

  • To review m6A modification regulatory proteins, including methyltransferases, demethylases, and methyl-binding proteins.
  • To elucidate the roles of m6A modification and its regulatory proteins in miRNA production and function across various biological contexts.
  • To discuss the intricate mechanisms by which miRNAs mediate m6A modification and its regulatory proteins in disease pathogenesis.

Main Methods:

  • Literature review of studies on m6A modification, regulatory proteins, and miRNAs.
  • Analysis of the interplay between m6A machinery and miRNA pathways.
  • Synthesis of current knowledge on m6A-miRNA interactions in physiological and pathological conditions.

Main Results:

  • Detailed summary of m6A methyltransferases, demethylases, and methyl-binding proteins.
  • Comprehensive overview of m6A's impact on miRNA production and function.
  • Exploration of miRNA-mediated regulatory mechanisms involving m6A and its proteins in disease.

Conclusions:

  • m6A modification and its regulatory proteins are crucial players in miRNA regulation.
  • Understanding these interactions provides insights into disease mechanisms.
  • This review consolidates knowledge and highlights future research directions in the m6A-miRNA field.
Keywords:
N6-metyladenosinedemethylasesmethyl-binding proteinsmethyltransferasesmiRNAs

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