Fluoroplast Doped by Ag₂O Nanoparticles as New Repairing Non-Cytotoxic Antibacterial Coating for Meat Industry

Foodborne infections are an important global health problem due to their high prevalence and potential for severe complications. Bacterial contamination of meat during processing at the enterprise can be a source of foodborne infections. Polymeric coatings with antibacterial properties can be applied to prevent bacterial contamination. A composite coating based on fluoroplast and Ag₂O NPs can serve as such a coating. In present study, we, for the first time, created a composite coating based on fluoroplast and Ag₂O NPs. Using laser ablation in water, we obtained spherical Ag₂O NPs with an average size of 45 nm and a ζ-potential of -32 mV. The resulting Ag₂O NPs at concentrations of 0.001-0.1% were transferred into acetone and mixed with a fluoroplast-based varnish. The developed coating made it possible to completely eliminate damage to a Teflon cutting board. The fluoroplast/Ag₂O NP coating was free of defects and inhomogeneities at the nano level. The fluoroplast/Ag₂O NP composite increased the production of ROS (H₂O₂, OH radical), 8-oxogualnine in DNA in vitro, and long-lived active forms of proteins. The effect depended on the mass fraction of the added Ag₂O NPs. The 0.01-0.1% fluoroplast/NP Ag₂O coating exhibited excellent bacteriostatic and bactericidal properties against both Gram-positive and Gram-negative bacteria but did not affect the viability of eukaryotic cells. The developed PTFE/NP Ag₂O 0.01-0.1% coating can be used to protect cutting boards from bacterial contamination in the meat processing industry.